Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
fusion protein of maltose binding protein and the N-terminal RNase H domain, to 2.2.5 A resolution. Protein is monomeric in solution but associates in the crystal to form a dimer | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | fusion protein of maltose binding protein and the N-terminal RNase H domain shows RNase H activity with a hybrid RNA/DNA substrate as well as double-stranded RNase activity. The full-length protein has additional CobC activity | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WLH5 | bifunctional protein, the N-terminal domain is homologous with prokaryotic and eukaryotic RNase H domains and the C-terminal domain with alpha-ribazole phosphatase CobC | - |
Mycobacterium tuberculosis H37Rv | P9WLH5 | bifunctional protein, the N-terminal domain is homologous with prokaryotic and eukaryotic RNase H domains and the C-terminal domain with alpha-ribazole phosphatase CobC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA duplex + H2O | substrate both for full-lentgh enzyme and isolated RNase H N-terminal RNase H domain | Mycobacterium tuberculosis | ? | - |
? | |
RNA-DNA duplex + H2O | substrate both for full-lentgh enzyme and isolated RNase H N-terminal RNase H domain | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
RNA-RNA duplex + H2O | substrate both for full-lentgh enzyme and isolated RNase H N-terminal RNase H domain | Mycobacterium tuberculosis | ? | - |
? | |
RNA-RNA duplex + H2O | substrate both for full-lentgh enzyme and isolated RNase H N-terminal RNase H domain | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rv2228c | - |
Mycobacterium tuberculosis |