Any feedback?
Literature summary for 3.1.26.4 extracted from
- A method for HLA genotyping using the specific cleavage of DNA-rN1-DNA/DNA with RNase HII from Chlamydia pneumoniae (2007), Oligonucleotides, 17, 433-443.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Chlamydia pneumoniae |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydia pneumoniae | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development and validation of a CpRNase HII-based method for activity assay and detection: DNA-rN1-DNA fragments are modified with a fluorophore at the 5'-end and a quencher at the 3'-end to generate molecular beacons, which hybridize with single-stranded DNA to be cleaved by CpRNase HII, the method is suitable for large-scale genotyping, overview | Chlamydia pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA-rN1-DNA/DNA + H2O | specific cleavage by RNase HII at the 5'-side of the ribonucleotide, cleavage efficiencies of the perfectly matched DNA-rN1-DNA/DNA duplexes are higher than those carrying a mismatched ribonucleotide | Chlamydia pneumoniae | ? | - |
? | |
| additional information | development of a CpRNase HII-based method for activity assay and detection: DNA-rN1-DNA fragments are modified with a fluorophore at the 5'-end and a quencher at the 3'-end to generate molecular beacons, which hybridize with single-stranded DNA to be cleaved by CpRNase HII, the method is suitable for large-scale genotyping, overview | Chlamydia pneumoniae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase HII | - |
Chlamydia pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Chlamydia pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Chlamydia pneumoniae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
