Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.4 extracted from

  • Lima, W.F.; Rose, J.B.; Nichols, J.G.; Wu, H.; Migawa, M.T.; Wyrzykiewicz, T.K.; Siwkowski, A.M.; Crooke, S.T.
    Human RNase H1 discriminates between subtle variations in the structure of the heteroduplex substrate (2007), Mol. Pharmacol., 71, 83-91.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged RNase H1 in Escherichia coli strain BL21(DE3) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information multiple-turnover kinetics with heteroduplexes Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-RNA hybrid + H2O Homo sapiens
-
DNA + 5'-phosphonucleotides
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged RNase H1 from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography followed by reverse phase chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-2'-methoxyethoxy-antisense RNA hybrid + H2O 2'-methoxyethoxy nucleotides, positioned at the 3' and 5' poles, into the antisense oligodeoxyribonucleotide of the heteroduplex to alter the helical geometry of the substrate Homo sapiens ?
-
?
DNA-RNA hybrid + H2O
-
Homo sapiens DNA + 5'-phosphonucleotides
-
?
additional information selective substrate recognition by RNase H1 Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
RNase H1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens