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Literature summary for 3.1.26.4 extracted from

  • Yarrington, R.M.; Chen, J.; Bolton, E.C.; Boeke, J.D.
    Mn2+ suppressor mutations and biochemical communication between Ty1 reverse transcriptase and RNase H domains (2007), J. Virol., 81, 9004-9012.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Protein Variants

Protein Variants Comment Organism
I183M site-directed mutagenesis in the polymerase domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition Saccharomyces cerevisiae
M520I site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition Saccharomyces cerevisiae
M520V site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition Saccharomyces cerevisiae
additional information generation of Ty1 reverse transcriptase/RNase H Mn2+ suppressor mutants capable of increased Ty1 transposition in pmr1DELTA cells for Mn2+ inhibition analysis, PMR1 codes for a P-type ATPase that regulates intracellular calcium and manganese ion homeostasis, and pmr1 mutants accumulate elevated intracellular manganese levels and display 100-fold less transposition than PMR1+ cells, suppressor point mutations localize not to the reverse transcriptase itself but to the RNase H domain of the protein, overview Saccharomyces cerevisiae
N398D site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition Saccharomyces cerevisiae
S392C site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition Saccharomyces cerevisiae
S442P site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition Saccharomyces cerevisiae
S469G site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition Saccharomyces cerevisiae
T467A site-directed mutagenesis in the connection domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition Saccharomyces cerevisiae
Y299C site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ Mn2+ inhibition of in vitro reverse transcriptase activity is greatly reduced in all the suppressor mutants, whereas RNAse H activity and cleavage specificity remain largely unchanged Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-RNA hybrid + H2O Saccharomyces cerevisiae in the pause of minus strang synthesis, RNAse H degrades the RNA template, with the exception of the polypurine tract sequence, immediately upstream of U3, which serves as a primer for plus-strand synthesis ssDNA + 5'-phosphomonoester oligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 strains JB740 and yEB104A
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid + H2O in the pause of minus strang synthesis, RNAse H degrades the RNA template, with the exception of the polypurine tract sequence, immediately upstream of U3, which serves as a primer for plus-strand synthesis Saccharomyces cerevisiae ssDNA + 5'-phosphomonoester oligonucleotides
-
 ?

Synonyms

Synonyms Comment Organism
RNase H
-
 Saccharomyces cerevisiae
Ty1 reverse transcriptase/RNase H
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Saccharomyces cerevisiae