Protein Variants | Comment | Organism |
---|---|---|
I183M | site-directed mutagenesis in the polymerase domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition | Saccharomyces cerevisiae |
M520I | site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition | Saccharomyces cerevisiae |
M520V | site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition | Saccharomyces cerevisiae |
additional information | generation of Ty1 reverse transcriptase/RNase H Mn2+ suppressor mutants capable of increased Ty1 transposition in pmr1DELTA cells for Mn2+ inhibition analysis, PMR1 codes for a P-type ATPase that regulates intracellular calcium and manganese ion homeostasis, and pmr1 mutants accumulate elevated intracellular manganese levels and display 100-fold less transposition than PMR1+ cells, suppressor point mutations localize not to the reverse transcriptase itself but to the RNase H domain of the protein, overview | Saccharomyces cerevisiae |
N398D | site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition | Saccharomyces cerevisiae |
S392C | site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition | Saccharomyces cerevisiae |
S442P | site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition | Saccharomyces cerevisiae |
S469G | site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition | Saccharomyces cerevisiae |
T467A | site-directed mutagenesis in the connection domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition | Saccharomyces cerevisiae |
Y299C | site-directed mutagenesis in the RNase H domain, the mutation results in a Mn2+ suppressor mutant less sensitive to Mn2+ inhibition | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | Mn2+ inhibition of in vitro reverse transcriptase activity is greatly reduced in all the suppressor mutants, whereas RNAse H activity and cleavage specificity remain largely unchanged | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | Saccharomyces cerevisiae | in the pause of minus strang synthesis, RNAse H degrades the RNA template, with the exception of the polypurine tract sequence, immediately upstream of U3, which serves as a primer for plus-strand synthesis | ssDNA + 5'-phosphomonoester oligonucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
strains JB740 and yEB104A | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | in the pause of minus strang synthesis, RNAse H degrades the RNA template, with the exception of the polypurine tract sequence, immediately upstream of U3, which serves as a primer for plus-strand synthesis | Saccharomyces cerevisiae | ssDNA + 5'-phosphomonoester oligonucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H | - |
Saccharomyces cerevisiae |
Ty1 reverse transcriptase/RNase H | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Saccharomyces cerevisiae |