Application | Comment | Organism |
---|---|---|
pharmacology | the enzyme is a potential target for antileishmanial chemotherapy | Leishmania donovani |
pharmacology | the enzyme is a potential target for antileishmanial chemotherapy | Leishmania major |
Cloned (Comment) | Organism |
---|---|
RNase HIIC, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3), genetic complementation of a RNase-deficient Escherichia coli strain fails due to lacking processing of the inactive precursor protein | Leishmania donovani |
RNase HIIC, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3), genetic complementation of a RNase-deficient Escherichia coli strain fails due to lacking processing of the inactive precursor protein | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | the enzyme contains a mitochondrial targeting sequence with the first 40 amino acid residues at the N-terminus, the enzyme is imported into mitochondrial vesicles in vitro, the enzyme is processed after entry into mitochondria | Leishmania donovani | 5739 | - |
mitochondrion | the enzyme contains a mitochondrial targeting sequence with the first 40 amino acid residues at the N-terminus, the enzyme is imported into mitochondrial vesicles in vitro, the enzyme is processed after entry into mitochondria | Leishmania major | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania donovani |
49000 | - |
x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania major |
53000 | - |
x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania donovani |
53000 | - |
x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leishmania donovani | the enzyme is required for kinetoplast DNA replication in the mitochondrion, the RNase HIIC is essential for growth of promastigotes and amastigotes | ? | - |
? | |
additional information | Leishmania major | the enzyme is required for kinetoplast DNA replication in the mitochondrion, the RNase HIIC is essential for growth of promastigotes and amastigotes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | Q8WR57 | single copy gene, 3 isozymes A, B, and C of the type II enzyme | - |
Leishmania major | Q8WSZ0 | single copy gene, 3 isozymes A, B, and C of the type II enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme is procesed after import into mitochondria | Leishmania donovani |
proteolytic modification | the enzyme is procesed after import into mitochondria | Leishmania major |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged RNAseHIIC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leishmania donovani |
recombinant His-tagged RNAseHIIC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leishmania major |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Leishmania donovani | - |
amastigote | - |
Leishmania major | - |
promastigote | - |
Leishmania donovani | - |
promastigote | - |
Leishmania major | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is required for kinetoplast DNA replication in the mitochondrion, the RNase HIIC is essential for growth of promastigotes and amastigotes | Leishmania donovani | ? | - |
? | |
additional information | the enzyme is required for kinetoplast DNA replication in the mitochondrion, the RNase HIIC is essential for growth of promastigotes and amastigotes | Leishmania major | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania donovani |
? | x * 53000, about, enzyme precursor, sequence calculation, x * 49000, processed enzyme, SDS-PAGE | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
LRNase HII | - |
Leishmania donovani |
LRNase HII | - |
Leishmania major |
type II ribonuclease H | - |
Leishmania donovani |
type II ribonuclease H | - |
Leishmania major |