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Literature summary for 3.1.26.4 extracted from

  • Fang, S.; Lee, H.J.; Wark, A.W.; Kim, H.M.; Corn, R.M.
    Determination of ribonuclease H surface enzyme kinetics by surface plasmon resonance imaging and surface plasmon fluorescence spectroscopy (2005), Anal. Chem., 77, 6528-6534.
    View publication on PubMed
Search for more literature for 3.1.26.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information determination of enzyme surface enzyme kinetics by surface plasmon resonance imaging and surface plasmon fluorescence spectroscopy, kinetic analysis, overview Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Escherichia coli
-

Storage Stability

Storage Stability Organism
-20°C, 25 mM Tris-HCl, pH 7.5, 30 mM NaCl, 0.5 mM EDTA, 5 mM 2-mercaptoethanol, 50% glycerol, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid + H2O
-
 Escherichia coli ?
-
 ?
DNA/RNA hybrid + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
ribonuclease H
-
 Escherichia coli
RNase H
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.95
-
 DNA/RNA hybrid pH 7.8-7.9, 25°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 7.9 assay at Escherichia coli