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Literature summary for 3.1.26.4 extracted from

  • Kitahara, N.; Sawai, Y.; Tsukada, K.
    Purification and properties of magnesium- and manganese-dependent ribonucleases H from chick embryo (1982), J. Biochem., 92, 855-864.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
sulfhydryl reagent required for maximal activity of the Mn2+-dependent enzyme and the Mg2+-dependent enzyme Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme Gallus gallus
diphosphate inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme Gallus gallus
NaF inhibition of Mg2+-dependent enzyme Gallus gallus
NEM 2 mM, 80% inhibition of Mn2+-dependent enzyme, complete inhibition of Mg2+-dependent enzyme Gallus gallus
PCMB 2 mM, complete inhibition of Mn2+-dependent enzyme and Mg2+-dependent enzyme Gallus gallus
spermidine inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme Gallus gallus
spermine inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+-dependent enzyme requires 15-20 mM Mg2+ for maximal activity Gallus gallus
Mg2+ optimal activity with 10-15 mM Gallus gallus
Mn2+ 0.4 mM Mn2+ required for optimal activity, some activity with Mg2+ Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
Mg2+-dependent enzyme, gel filtration Gallus gallus
120000
-
Mn2+-dependent enzyme, gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
one Mn2+-dependent RNase H and one Mg2+-dependent RNase H
-

Purification (Commentary)

Purification (Comment) Organism
one Mn2+-dependent RNase H and one Mg2+-dependent RNase H Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA hybrid + H2O
-
Gallus gallus ribonucleotide 5'-phosphomonoester a mixture of oligoribonucleotides with 5'-phosphate and 3'-hydroxyl terminus ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
Mn2+-dependent enzyme Gallus gallus
9.5
-
Mg2+-dependent enzyme Gallus gallus