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Literature summary for 3.1.26.4 extracted from
- Purification and properties of two ribonuclease H enzymes from yeast (1982), Arch. Biochem. Biophys., 219, 110-120.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NEM | 0.03 mM, 50% inhibition after 30 min | Saccharomyces cerevisiae |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement for Mg2+, optimal activity at 2-6 mM MgCl2 | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the apparent higher molecular weight obtained by gel filtration compared to sucrose density gradient centrifugation is probably caused by asymmetry of the enzyme protein | Saccharomyces cerevisiae |
| 67000 | - |
sucrose density gradient centrifugation | Saccharomyces cerevisiae |
| 110000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
ribonuclease HA | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA-DNA hybrid + H2O | poly(rUdA) | Saccharomyces cerevisiae | ribonucleotide 5'-phosphomonoester | 5'-phosphorylated oligonucleotides | ? | |
| RNA-DNA hybrid + H2O | poly(rCdI) | Saccharomyces cerevisiae | ribonucleotide 5'-phosphomonoester | 5'-phosphorylated oligonucleotides | ? | |
| RNA-DNA hybrid + H2O | poly(rAdT) | Saccharomyces cerevisiae | ribonucleotide 5'-phosphomonoester | 5'-phosphorylated oligonucleotides | ? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
50% loss of activity after 45 s | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | unstable below pH 5.0 and above pH 8.0 | Saccharomyces cerevisiae |
| 6 | 7 | most stable at | Saccharomyces cerevisiae |
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