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Literature summary for 3.1.21.5 extracted from
- DNA base flipping by both members of the PspGI restriction-modification system (2008), Nucleic Acids Res., 36, 5417-5425.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain ER2744 | Pyrococcus sp. GI-H |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D138A | catalytically inactive | Pyrococcus sp. GI-H |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Pyrococcus sp. GI-H |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus sp. GI-H | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| P11 cellulose phosphate column chromatography | Pyrococcus sp. GI-H |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Pyrococcus sp. GI-H | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PspGI | recognizes the sequence CCWGG where W is A or T and cuts DNA before the first C in the cognate sequence | Pyrococcus sp. GI-H |
| REase | - |
Pyrococcus sp. GI-H |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
the optimal temperature for DNA cleavage by PspGI is at 80°C or above | Pyrococcus sp. GI-H |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
half life of 2 h at 95°C | Pyrococcus sp. GI-H |
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Release 2023.1 (January 2023)
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