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Literature summary for 3.1.21.4 extracted from

  • Ishikawa, K.; Watanabe, M.; Kuroita, T.; Uchiyama, I.; Bujnicki, J.M.; Kawakami, B.; Tanokura, M.; Kobayashi, I.
    Discovery of a novel restriction endonuclease by genome comparison and application of a wheat-germ-based cell-free translation assay: PabI (5'-GTA/C) from the hyperthermophilic archaeon Pyrococcus abyssi (2005), Nucleic Acids Res., 33, e112.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.4

Application

Application Comment Organism
molecular biology tools for the dissection, analysis and reconstruction of DNA Pyrococcus abyssi

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrococcus abyssi

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl optimally active at NaCl concentrations ranging from 100-200 mM Pyrococcus abyssi

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi G8ZFZ3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus abyssi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the enzyme recognizes 5'-GTAC and leaves a 3'-TA overhang (5'-GTA/C) Pyrococcus abyssi double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Synonyms

Synonyms Comment Organism
PabI
-
 Pyrococcus abyssi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Pyrococcus abyssi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 1 h, the enzyme retains approximately 50% of its activity Pyrococcus abyssi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pyrococcus abyssi