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Literature summary for 3.1.21.4 extracted from

  • Morgan, R.D.; Bhatia, T.K.; Lovasco, L.; Davis, T.B.
    MmeI: a minimal Type II restriction-modification system that only modifies one DNA strand for host protection (2008), Nucleic Acids Res., 36, 6558-6570.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.4

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine MmeI endonuclease activity requires S-adenosyl-L-methionine Methylophilus methylotrophus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain ER2683 Methylophilus methylotrophus

Inhibitors

Inhibitors Comment Organism Structure
additional information MmeI endonuclease is sensitive only to top strand methylation Methylophilus methylotrophus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MmeI endonuclease activity requires magnesium ions Methylophilus methylotrophus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105100
-
 calculated from amino acid sequence Methylophilus methylotrophus

Organism

Organism UniProt Comment Textmining
Methylophilus methylotrophus B2MU09
-
-

Purification (Commentary)

Purification (Comment) Organism
heparin column chromatography, Source Q column chromatography, Source S column chromatography, Superdex 75 gel filtration, and ceramic HTP column chromatography Methylophilus methylotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O MmeI cuts DNA two turns of the helix away from its asymmetric recognition sequence, 5'-TCCRACN20/N18-3', MmeI modifies only the adenine in the top strand, 5'-TCCRAC-3', MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5'-GTYGGA-3', MmeI methylates its recognition site following DNA cleavage Methylophilus methylotrophus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
additional information the head-to-head configuration substrate, pUC19HH1, is digested both as closed circular DNA and linear DNA Methylophilus methylotrophus ?
-
 ?

Synonyms

Synonyms Comment Organism
MmeI
-
 Methylophilus methylotrophus
type II restriction enzyme
-
 Methylophilus methylotrophus