Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K263A | inactive mutant with truncated EcoRII N-domain | Escherichia coli |
R330A | inactive mutant with truncated EcoRII C-domain | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | in the presence of Ca2+ ions, EcoRII binds to the target DNA but does not cleave the DNA strand | Escherichia coli | |
Mg2+ | required | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap heparin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | EcoRII recognizes two units of recognition sequences (5'-CCWGG-3') included in one DNA chain (cis-binding) or in two DNA chains one by one (trans-binding), and cleaves either site | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
additional information | no activity is observed using 1-site DNA as substrate | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
EcoRII | - |
Escherichia coli |
type IIE restriction endonuclease | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
DNA | wild type enzyme with 2-site DNA | Escherichia coli |