Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BNH670 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L80P | the mutant enzyme shows decreased DNA methyltransferase activity at a higher temperature in vivo and in vitro than the wild type enzyme, the activity of the L80P mutant is completely lost at a high temperature | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P14633 | - |
- |
Escherichia coli BNH2586 | P14633 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
DNA + H2O | - |
Escherichia coli BNH2586 | double-stranded DNA fragments with terminal 5'-phosphates | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EcoRII | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 42 | the extract from cells expressing the wild type EcoRII shows the activity both at 30°C and 37°C, the extract from cells expressing the L80P mutant form shows activity at 30°C but not at 37°C, the L80P mutant protein is significantly unstable at 42°C compared with the wild type protein | Escherichia coli |