Literature summary for 3.1.21.4 extracted from

Doyon, J.B.; Pattanayak, V.; Meyer, C.B.; Liu, D.R.
Directed evolution and substrate specificity profile of homing endonuclease I-SceI (2006), J. Am. Chem. Soc., 128, 2477-2484.

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Application

Application	Comment	Organism
biotechnology	evolvement of mutant enzymes with altered DNA cleavage specificities by application of an in vivo positive and negative selection system that applies evolutionary pressure either to favor the cleavage of a desired target sequence or to disfavor the cleavage of a nontarget sequence	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	evolvement of mutant enzymes with altered DNA cleavage specificities by application of an in vivo positive and negative selection system that applies evolutionary pressure either to favor the cleavage of a desired target sequence or to disfavor the cleavage of a nontarget sequence	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	isoform Sce-I	-

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Release 2023.1 (January 2023)