Literature summary for 3.1.21.4 extracted from

Senejani, A.G.; Gogarten, J.P.
Structural stability and endonuclease activity of a PI-SceI GFP-fusion protein (2007), Int. J. Biol. Sci., 3, 205-211.

Search for more literature for 3.1.21.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escheichia coli, induction at 15°C results in accumulation in the cytoplasm, induction at 20-37°C results in formation of inclusion bodies	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme is an endonuclease encoded as a protein insert or intein within the yeast V-ATPase catalytic subunit encoding gene vma1. Insertion of the Green Fluorescence Protein into a loop which is located between the endonuclease and splicing domains of the Sce VMA1 intein. The GFP is functional and the additional GFP domain does not prevent intein excision and endonuclease activity. Contrary to wild-type, mutant requires the presence of Mn2+ and not Mg2+ ions for activity	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for wild-type activity	Saccharomyces cerevisiae
Mn2+	required for activity of mutant which carries an insertion of the Green Fluorescence Protein into a loop which is located between the endonuclease and splicing domains of the Sce VMA1 intein	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	isoform SceI	-

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Release 2023.1 (January 2023)