Protein Variants | Comment | Organism |
---|---|---|
D299A | no catalytic activity, mutant like wild-type remains in dimeric form | Escherichia coli |
D329A | less than 0.01% residual activity, mutant like wild-type remains in dimeric form | Escherichia coli |
E271A | no catalytic activity, mutant like wild-type remains in dimeric form | Escherichia coli |
E337A | less than 0.01% residual activity, mutant like wild-type remains in dimeric form | Escherichia coli |
K324A | no catalytic activity, mutant like wild-type remains in dimeric form | Escherichia coli |
K328A | less than 0.01% residual activity, mutant like wild-type remains in dimeric form | Escherichia coli |
R330A | no catalytic activity, mutant like wild-type remains in dimeric form | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, calculated | Escherichia coli |
54000 | 57000 | gel-filtration, wild-type and mutants | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isoform EcoRII | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Escherichia coli | ? | the isolated C-terminal domain dimer has an interface that binds a single cognate DNA molecule whereas the N-terminal domain is a monomer that also binds a single copy of cognate DNA | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, calculated | Escherichia coli |
More | the isolated C-terminal domain dimer has an interface that binds a single cognate DNA molecule whereas the N-terminal domain is a monomer that also binds a single copy of cognate DNA | Escherichia coli |