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Literature summary for 3.1.21.4 extracted from

  • Gowers, D.M.; Bellamy, S.R.; Halford, S.E.
    One recognition sequence, seven restriction enzymes, five reaction mechanisms (2004), Nucleic Acids Res., 32, 3469-3479.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Brevibacillus brevis
-
-
-
Kluyvera ascorbata
-
-
-
Micrococcus lylae
-
-
-
Nocardia argentinensis
-
-
-
Pantoea agglomerans
-
-
-
Pluralibacter gergoviae
-
-
-
Serratia fonticola
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O cleavage at 5'-G-/-GCGCC-3'. The enzyme cuts only one bond per turnover but acts at individual sites, preferring intact to nicked sites Kluyvera ascorbata double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O cleavage at 5'-GG-/-CGCC-3'. The enzyme binds two sites, but cleaves only one bond per DNA-binding event Nocardia argentinensis double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O cleavage at 5'-GG-/-CGCC-3'. The enzyme cuts both strands of its recognition sites, but shows full activity only when bound to two sites, which are then cleaved concertedly Micrococcus lylae double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O cleavage at 5'-GGC-/-GCC-3'. The enzyme cuts both strands at individual sites Pantoea agglomerans double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O cleavage at 5'-GGC-/-GCC-3'. The enzyme cuts both strands at individual sites Serratia fonticola double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O cleavage at 5'-GGC-/-GCC-3'. The enzyme cuts both strands at individual sites Pluralibacter gergoviae double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O cleavage at 5'-GGCGC-/-C-3. The enzyme displays an absolute requirement for two sites in close physical proximity, which are cleaved concertedly Brevibacillus brevis double-stranded DNA fragments with terminal 5'-phosphates
-
?

Synonyms

Synonyms Comment Organism
BbeI
-
Brevibacillus brevis
EgeI
-
Pluralibacter gergoviae
EheI
-
Pantoea agglomerans
KasI
-
Kluyvera ascorbata
Mly113I
-
Micrococcus lylae
NarI
-
Nocardia argentinensis
SfoI
-
Serratia fonticola