Protein Variants | Comment | Organism |
---|---|---|
additional information | mutants of BbvCI with defects in one subunit, either R1-R2+ or R1+R2-, cleave only one strand of the 5'-CC-/-TCAGC-3'/5'-GC-/-TGAGG-3' sequence | Brevibacillus brevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus brevis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | BbvCI cleaves the assymetric DNA sequence, 5'-CC-/-TCAGC-3'/5'-GC-/-TGAGG-3'. The R1 subunit of the enzyme acts at GC-/-TGAGG and the R2 subunit acts at CC-/-TCAGC. the DNA is cleaved initially in one strand, mainly that targeted by the R1 subunit. The other strand is then cleaved slowly by R2 before the enzyme dissociates from the DNA | Brevibacillus brevis | double-stranded DNA fragments with terminal 5'-phosphates | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BbvCI | - |
Brevibacillus brevis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
DNA | 37°C, R1+-R2- mutant | Brevibacillus brevis | |
0.0037 | - |
DNA | 37°C, R1-R2+ mutant | Brevibacillus brevis | |
0.048 | - |
DNA | 37°C, wild-type enzyme | Brevibacillus brevis |