Protein Variants | Comment | Organism |
---|---|---|
DELTA362-465 | nicking variant of BsaI, nicking occurs on top strand | Geobacillus stearothermophilus |
DELTA440-544 | nicking variant of BsaI, nicking occurs on bottom strand | Geobacillus stearothermophilus |
DELTA446-544 | nicking variant of BsaI | Geobacillus stearothermophilus |
K150R/R236G | nicking variant of BsaI, nicking occurs on top strand | Geobacillus stearothermophilus |
N349Y | nicking variant of BsmAI, nicking occurs on top strand | Geobacillus stearothermophilus |
N415D/R416G | variant of BsmAI, both nicking activity and dsDNA cleavage | Geobacillus stearothermophilus |
N441D | variant of BsaI, cleavage of dsDNA | Geobacillus stearothermophilus |
N441D/R442G | nicking variant of BsaI, nicking occurs on bottom strand | Geobacillus stearothermophilus |
R221D | nicking variant of BsmAI, nicking on top strand | Geobacillus stearothermophilus |
R233D | nicking variant of BsmBI, nicking on top strand, about 5-10% cleavage of dsDNA | Geobacillus stearothermophilus |
R236D | nicking variant of BsaI, nicking on top strand, no cleavage of dsDNA | Geobacillus stearothermophilus |
R236G | nicking variant of BsaI, 5-10% cleavage of dsDNA | Geobacillus stearothermophilus |
R386S | nicking variant of BsaI, nicking occurs on top strand | Geobacillus stearothermophilus |
R438D | nicking variant of BsmBI, nicking on bottom strand | Geobacillus stearothermophilus |
R438G | nicking variant of BsmBI | Geobacillus stearothermophilus |
R442G | nicking variant of BsaI, 5% cleavage of dsDNA | Geobacillus stearothermophilus |
S128L/R236G | nicking variant of BsaI, nicking occurs on top strand | Geobacillus stearothermophilus |
W8C/G207E | nicking variant of BsaI, nicking occurs on top strand | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
enzymes BsaI, BsmBI, BsmAI | - |