Application | Comment | Organism |
---|---|---|
additional information | inactivation of the hsdR gene of the Sau1 type I restriction-modification system is responsible for the high transformation efficiency of RN4220 | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
hsdR PCR product cloned into pMAD, giving rise to pDELTAHsdR-1. Plasmid electroporated into the transformable strain RN4220 at 30°C, with erythromycin selection, and subsequently transduced to NCTC8325-4, SH1000 and COL using phage 80alpha | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | hsdR null mutants, complete deletion of the hsdR gene is not sufficient to generate readily transformable NCTC8325-4, SH1000 and COL strains. HsdR mutant strains have growth rates identical to the parental strains and and are competent if transformed with appropriately modified Staphylococcus aureus DNA. HsdR mutants are competent but do not accept foreign DNA due to the presence of additional factor(s) that degrade unmodified DNA. Heat treatment of competent cells increases transformation efficiency of hsdR mutants | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
strain NCTC8325-4, strain SH1000, strain COL and strain 879R4RF | - |
Synonyms | Comment | Organism |
---|---|---|
Sau1 type I restriction-modification system | - |
Staphylococcus aureus |