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Literature summary for 3.1.21.2 extracted from

  • Hirano, N.; Ohshima, H.; Sakashita, H.; Takahashi, H.
    The Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli (2007), Nucleic Acids Res., 35, 6692-6700.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-Endo IV fusion protein in Escherichia coli: wild-type and mutants W88R and S176N Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
S176N mutant enzyme retains cleavage activity (17.5% of that of wild-type Endo IV), but loses the polarized and restricted cleavage of a dCs tract. Escherichia coli cells expressing the intact Endo IV mutant enzyme are viable and, in contrast to wild-type Endo IV, the mutant enzyme does not show detrimental effect on the host cells Tequatrovirus T4
W88R mutant enzyme shows no enzymatic activity (less than 0.4% of that of wild-type Endo IV). Escherichia coli cells expressing the intact Endo IV mutant enzyme are viable and, in contrast to wild-type Endo IV, these mutant enzymes do not show detrimental effect on the host cells Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values of the wild-type and S176N mutant forms of Endo IV are determined with series of 25-base oligonucleotides Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
single-stranded DNA + H2O Tequatrovirus T4 the Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli 5'-phosphooligonucleotides
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 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
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-
-

Purification (Commentary)

Purification (Comment) Organism
GST-Endo IV fusion proteins: wild-type and mutants W88R and S176N Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
single-stranded DNA + H2O the Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli Tequatrovirus T4 5'-phosphooligonucleotides
-
 ?
single-stranded DNA + H2O endonuclease IV cleaves single-stranded (ss) DNA in a dC-specific manner. The Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli Tequatrovirus T4 5'-phosphooligonucleotides
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 ?

Synonyms

Synonyms Comment Organism
endonuclease IV
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 Tequatrovirus T4