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Literature summary for 3.1.21.2 extracted from

  • Ohshima, H.; Hirano, N.; Takahashi, H.
    A hexanucleotide sequence (dC1-dC6 tract) restricts the dC-specific cleavage of single-stranded DNA by endonuclease IV of bacteriophage T4 (2007), Nucleic Acids Res., 35, 6681-6689.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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 additional information Km-values of Endo IV with with oligonucleotide substrates containing a target sequence and with various dC1-dC6 tracts and the effects of third dC residue in the tract Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
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Purification (Commentary)

Purification (Comment) Organism
GST-Endo IV fusion protein Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
single-stranded DNA + H2O the enzyme cleaves single-stranded (ss) DNA in a dC-specific manner. Endo IV cleaves ssDNAs exclusively at the 5’-proximal dC where a sequence comprises dC residues both at the 5’ proximal and 3’ proximal positions (a dCs tract-dependent cleavage). The dCs tract-dependent cleavage is efficient and occurs when a dCs tract has at least 6 bases. Some dCs tracts larger than 6 bases behave as that of 6 bases (an extended dCs tract), while some others do not. One decameric dCs tract is shown to be cleavable in a dCs tract-dependent manner, but that with 13 dCs is not. The dCs tract-dependent cleavage is enhanced by the presence of a third dC residue at least for a 6 or 7 dCs tract. In contrast to the dCs tract-dependent cleavage, a dCs tract-independent one is generally inefficient and if two modes are possible for a substrate DNA, a dCs tract-dependent mode prevails Tequatrovirus T4 5'-phosphooligonucleotides
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