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Literature summary for 3.1.21.2 extracted from

  • Hirano, N.; Ohshima, H.; Takahashi, H.
    Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis (2006), Nucleic Acids Res., 34, 4743-4751.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information effects of dC-flanking sequences of the substrate on kinetic parameters of Endo IV Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Tequatrovirus T4 endonuclease IV encoded by denB of bacteriophage T4 is implicated in restriction of deoxycytidine (dC)-containing DNA in the host Escherichia coli ?
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Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information endonuclease IV encoded by denB of bacteriophage T4 is implicated in restriction of deoxycytidine (dC)-containing DNA in the host Escherichia coli Tequatrovirus T4 ?
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 ?
single-stranded DNA + H2O Endo IV catalyzes specific endonucleolytic cleavage of the 5' phosphodiester bond of dC in ssDNA with an efficiency dependent on the surrounding nucleotide sequence. The enzyme preferentially targets 5'-dTdCdA-3' but tolerates various combinations of individual nucleotides flanking this trinucleotide sequence. Endo IV preferentially recognizes short nucleotide sequences containing 5'-TdCdA-3', which likely accounts for the limited digestion of ssDNA by the enzyme and may be responsible in part for the indispensability of a deficiency in denB for stable synthesis of dC-substituted T4 genomic DNA Tequatrovirus T4 5'-phosphooligonucleotides
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 ?

Synonyms

Synonyms Comment Organism
endonuclease IV
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 Tequatrovirus T4

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information effects of dC-flanking sequences of the substrate on kinetic parameters of Endo IV Tequatrovirus T4