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Literature summary for 3.1.21.1 extracted from

  • Chen, W.J.; Liao, T.H.
    2-Nitro-5-thiosulfobenzoic acid as a novel inhibitor specific for deoxyribonuclease I (2008), Protein J., 27, 240-246.
    View publication on PubMed
Search for more literature for 3.1.21.1

Inhibitors

Inhibitors Comment Organism Structure
2-nitro-5-thiosulfobenzoic acid the presence of Ca2+ or Mg2+ at pH 7.5 results in 80% inactivation without fragmentation of the enzyme. In the absence of metal ions it retains 80% of its activity. It binds DNase I through covalent modification, since dialysis and gel filtration can not reverse the inactivation reaction. After dilution into an acid buffer of pH 4.7, the inactivated enzyme regains about 40% of its initial activity. The inhibitor fails to inactivate other enzymes, suggesting that the inhibition is unique to DNase I Bos taurus
2-nitro-5-thiosulfobenzoic acid is a novel inhibitor specific for DNase I Rattus norvegicus
additional information DTNB or Na2SO3 alone do not inactivate DNase I, even in the presence of divalent cations Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ in the presence of Ca2+, native (0.2 U) and inactivated DNase I of 5fold greater enzyme units applied (1.0 U) hydrolyze the Mg2+-DNA substrate forming some linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission, while 0.2 U of 2-nitro-5-thiosulfobenzoic acid-treated DNase I can only cleave the Mg2+-DNA substrate in a single nicking mode Bos taurus
Mg2+ in the absence of Ca2+, only native DNase I cleaves the Mg2+-DNA substrate in a single nicking mode with the formation of only the relaxed open-circular DNA, while the 2-nitro-5-thiosulfobenzoic acid-treated DNase I fails to cleave the plasmid DNA substrate Bos taurus
Mn2+ in the presence of Mn2+, native and 2-nitro-5-thiosulfobenzoic acid-treated DNase I of two different enzyme units used are all able to hydrolyze the plasmid DNA substrate in a double scission mode Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-
parotid gland
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
plasmid DNA + H2O depending on metal ions, duplex DNA is hydrolyzed by DNase I in a single or double scission mode Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
DNase I
-
 Rattus norvegicus
DNase I
-
 Bos taurus