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Literature summary for 3.1.21.1 extracted from

  • Schernthaner, J.P.; Milne, R.E.; Kaplan, H.
    Characterization of a novel insect digestive DNase with a highly alkaline pH optimum (2002), Insect Biochem. Mol. Biol., 32, 255-263.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Choristoneura fumiferana
K+ no inhibition at 50 mM, 50% inhibition at 200 mM Choristoneura fumiferana
Na+ no inhibition at 50 mM, 50% inhibition at 200 mM Choristoneura fumiferana

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Choristoneura fumiferana
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, transition state metal ions also activate, but are less effective Choristoneura fumiferana
Mg2+ best activator, transition state metal ions also activate, but are less effective Choristoneura fumiferana
additional information divalent alkaline metal ion requirement Choristoneura fumiferana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
x * 23000, SDS-PAGE Choristoneura fumiferana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dsDNA + H2O Choristoneura fumiferana
-
5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
?
additional information Choristoneura fumiferana enzyme is an integral component of the larval digestive system, enzyme plays an important role in the conversion of the insecticidal crystal protein from Bacillus thuringiensis to the active DNA-free toxin in the larval gut, purified enzyme acts synergistically with the toxin ?
-
?
ssDNA + H2O Choristoneura fumiferana
-
5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
?

Organism

Organism UniProt Comment Textmining
Choristoneura fumiferana
-
spruce budworm
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Choristoneura fumiferana

Purification (Commentary)

Purification (Comment) Organism
from larval gut Choristoneura fumiferana

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products preference for single-stranded DNA Choristoneura fumiferana

Source Tissue

Source Tissue Comment Organism Textmining
gut
-
Choristoneura fumiferana
-
larva
-
Choristoneura fumiferana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + H2O
-
Choristoneura fumiferana 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
?
additional information no activity with homo-oligomers of dA, dC, dG and annealed homo-oligomers of dA/dT, no activity with RNA Choristoneura fumiferana ?
-
?
additional information enzyme is an integral component of the larval digestive system, enzyme plays an important role in the conversion of the insecticidal crystal protein from Bacillus thuringiensis to the active DNA-free toxin in the larval gut, purified enzyme acts synergistically with the toxin Choristoneura fumiferana ?
-
?
ssDNA + H2O
-
Choristoneura fumiferana 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
?
ssDNA + H2O preferred substrate Choristoneura fumiferana 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE Choristoneura fumiferana

Synonyms

Synonyms Comment Organism
DNase I
-
Choristoneura fumiferana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5 11
-
Choristoneura fumiferana

pH Range

pH Minimum pH Maximum Comment Organism
9.5 12 pH activity profile Choristoneura fumiferana