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Literature summary for 3.1.21.1 extracted from

  • Desai, N.A.; Shankar, V.
    Purification and characterization of the single-strand-specific and guanylic-acid-preferential deoxyribonuclease activity of the extracellular nuclease from Basidiobolus haptosporus (2000), Eur. J. Biochem., 267, 5123-5135.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 70% inhibition at 1 mM Basidiobolus haptosporus
3'-AMP
-
Basidiobolus haptosporus
5'-deoxy-GMP competitive, product inhibition Basidiobolus haptosporus
5'-GDP
-
Basidiobolus haptosporus
5'-GMP
-
Basidiobolus haptosporus
5'-GTP
-
Basidiobolus haptosporus
Ag2+
-
Basidiobolus haptosporus
Al3+
-
Basidiobolus haptosporus
diphosphate
-
Basidiobolus haptosporus
DTT 80% inhibition at 1 mM Basidiobolus haptosporus
Fe3+
-
Basidiobolus haptosporus
guanidinium hydrochloride over 80% inhibition at 0.5 M Basidiobolus haptosporus
Hg2+
-
Basidiobolus haptosporus
KCl stimulating between 25 and 50 mM, inhibitory above 50 mM Basidiobolus haptosporus
additional information the phosphate residue is responsible for the inhibitory effect of guanosine 5'-nucleotides Basidiobolus haptosporus
NaCl stimulating between 25 and 50 mM, inhibitory above 50 mM Basidiobolus haptosporus
phosphate
-
Basidiobolus haptosporus
SDS over 80% inhibition at 0.04% w/v Basidiobolus haptosporus
Urea 20% inhibition at 4 M Basidiobolus haptosporus
Zn2+
-
Basidiobolus haptosporus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Basidiobolus haptosporus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl stimulating between 25 and 50 mM, inhibitory above 50 mM Basidiobolus haptosporus
additional information metalloprotein, enzyme has no obligate requirement for metal ions to be active, nor is its activity stimulated by addition of metal ions Basidiobolus haptosporus
NaCl stimulating between 25 and 50 mM, inhibitory above 50 mM Basidiobolus haptosporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
1 * 30000, deglycosylated enzyme, SDS-PAGE, 42000-47000, glycosylated enzyme, SDS-PAGE Basidiobolus haptosporus
41000
-
gel filtration Basidiobolus haptosporus

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl formamide 20% decrease in activity at 40% v/v Basidiobolus haptosporus
dimethyl sulfoxide 20% decrease in activity at 15% v/v Basidiobolus haptosporus
formamide stable to at 50% v/v Basidiobolus haptosporus

Organism

Organism UniProt Comment Textmining
Basidiobolus haptosporus
-
multifunctional enzyme
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 15% carbohydrate content Basidiobolus haptosporus

Purification (Commentary)

Purification (Comment) Organism
6057fold to homogeneity Basidiobolus haptosporus

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products preference for single-stranded DNA and 3'AMP, guanylic acid preferential endo-exonuclease, mode of action, overview Basidiobolus haptosporus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14540
-
purified enzyme, substrate RNA Basidiobolus haptosporus
597200
-
purified enzyme, substrate ssDNA Basidiobolus haptosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'AMP + H2O phosphomonoesterase activity Basidiobolus haptosporus adenosine + phosphate
-
?
dsDNA + H2O very low activity Basidiobolus haptosporus ?
-
?
additional information substrate specificity, 5'-AMP, bis(4-nitrophenyl)phosphate, and 4-nitrophenyl phosphate are no substrates, preferential liberation of 5'-deoxy-GMP, no cleavage of C-linkages, (dC)10 is no substrate, no liberation of 5'-deoxy-CMP Basidiobolus haptosporus ?
-
?
phage M13 DNA + H2O endolytically cleavage generating single base nicks Basidiobolus haptosporus ?
-
?
RNA + H2O low activity Basidiobolus haptosporus ?
-
?
ssDNA + H2O
-
Basidiobolus haptosporus ?
-
?
supercoiled pUC18 DNA + H2O endolytically cleavage generating single base nicks Basidiobolus haptosporus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000, deglycosylated enzyme, SDS-PAGE, 42000-47000, glycosylated enzyme, SDS-PAGE Basidiobolus haptosporus

Synonyms

Synonyms Comment Organism
More see also EC 3.1.30.1 Basidiobolus haptosporus
nuclease Bh1
-
Basidiobolus haptosporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Basidiobolus haptosporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
15 min, stable Basidiobolus haptosporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Basidiobolus haptosporus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0028
-
5'-GTP pH 8.5, 37°C Basidiobolus haptosporus
0.0058
-
5'-GDP pH 8.5, 37°C Basidiobolus haptosporus
0.0283
-
5'-GMP pH 8.5, 37°C Basidiobolus haptosporus
0.04
-
5'-deoxy-GMP pH 8.5, 37°C Basidiobolus haptosporus

pI Value

Organism Comment pI Value Maximum pI Value
Basidiobolus haptosporus isoelectric focusing
-
6.8