Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 70% inhibition at 1 mM | Basidiobolus haptosporus | |
3'-AMP | - |
Basidiobolus haptosporus | |
5'-deoxy-GMP | competitive, product inhibition | Basidiobolus haptosporus | |
5'-GDP | - |
Basidiobolus haptosporus | |
5'-GMP | - |
Basidiobolus haptosporus | |
5'-GTP | - |
Basidiobolus haptosporus | |
Ag2+ | - |
Basidiobolus haptosporus | |
Al3+ | - |
Basidiobolus haptosporus | |
diphosphate | - |
Basidiobolus haptosporus | |
DTT | 80% inhibition at 1 mM | Basidiobolus haptosporus | |
Fe3+ | - |
Basidiobolus haptosporus | |
guanidinium hydrochloride | over 80% inhibition at 0.5 M | Basidiobolus haptosporus | |
Hg2+ | - |
Basidiobolus haptosporus | |
KCl | stimulating between 25 and 50 mM, inhibitory above 50 mM | Basidiobolus haptosporus | |
additional information | the phosphate residue is responsible for the inhibitory effect of guanosine 5'-nucleotides | Basidiobolus haptosporus | |
NaCl | stimulating between 25 and 50 mM, inhibitory above 50 mM | Basidiobolus haptosporus | |
phosphate | - |
Basidiobolus haptosporus | |
SDS | over 80% inhibition at 0.04% w/v | Basidiobolus haptosporus | |
Urea | 20% inhibition at 4 M | Basidiobolus haptosporus | |
Zn2+ | - |
Basidiobolus haptosporus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Basidiobolus haptosporus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | stimulating between 25 and 50 mM, inhibitory above 50 mM | Basidiobolus haptosporus | |
additional information | metalloprotein, enzyme has no obligate requirement for metal ions to be active, nor is its activity stimulated by addition of metal ions | Basidiobolus haptosporus | |
NaCl | stimulating between 25 and 50 mM, inhibitory above 50 mM | Basidiobolus haptosporus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 30000, deglycosylated enzyme, SDS-PAGE, 42000-47000, glycosylated enzyme, SDS-PAGE | Basidiobolus haptosporus |
41000 | - |
gel filtration | Basidiobolus haptosporus |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl formamide | 20% decrease in activity at 40% v/v | Basidiobolus haptosporus |
dimethyl sulfoxide | 20% decrease in activity at 15% v/v | Basidiobolus haptosporus |
formamide | stable to at 50% v/v | Basidiobolus haptosporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Basidiobolus haptosporus | - |
multifunctional enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 15% carbohydrate content | Basidiobolus haptosporus |
Purification (Comment) | Organism |
---|---|
6057fold to homogeneity | Basidiobolus haptosporus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | preference for single-stranded DNA and 3'AMP, guanylic acid preferential endo-exonuclease, mode of action, overview | Basidiobolus haptosporus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14540 | - |
purified enzyme, substrate RNA | Basidiobolus haptosporus |
597200 | - |
purified enzyme, substrate ssDNA | Basidiobolus haptosporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'AMP + H2O | phosphomonoesterase activity | Basidiobolus haptosporus | adenosine + phosphate | - |
? | |
dsDNA + H2O | very low activity | Basidiobolus haptosporus | ? | - |
? | |
additional information | substrate specificity, 5'-AMP, bis(4-nitrophenyl)phosphate, and 4-nitrophenyl phosphate are no substrates, preferential liberation of 5'-deoxy-GMP, no cleavage of C-linkages, (dC)10 is no substrate, no liberation of 5'-deoxy-CMP | Basidiobolus haptosporus | ? | - |
? | |
phage M13 DNA + H2O | endolytically cleavage generating single base nicks | Basidiobolus haptosporus | ? | - |
? | |
RNA + H2O | low activity | Basidiobolus haptosporus | ? | - |
? | |
ssDNA + H2O | - |
Basidiobolus haptosporus | ? | - |
? | |
supercoiled pUC18 DNA + H2O | endolytically cleavage generating single base nicks | Basidiobolus haptosporus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, deglycosylated enzyme, SDS-PAGE, 42000-47000, glycosylated enzyme, SDS-PAGE | Basidiobolus haptosporus |
Synonyms | Comment | Organism |
---|---|---|
More | see also EC 3.1.30.1 | Basidiobolus haptosporus |
nuclease Bh1 | - |
Basidiobolus haptosporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Basidiobolus haptosporus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
15 min, stable | Basidiobolus haptosporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Basidiobolus haptosporus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
5'-GTP | pH 8.5, 37°C | Basidiobolus haptosporus | |
0.0058 | - |
5'-GDP | pH 8.5, 37°C | Basidiobolus haptosporus | |
0.0283 | - |
5'-GMP | pH 8.5, 37°C | Basidiobolus haptosporus | |
0.04 | - |
5'-deoxy-GMP | pH 8.5, 37°C | Basidiobolus haptosporus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Basidiobolus haptosporus | isoelectric focusing | - |
6.8 |