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Literature summary for 3.1.21.1 extracted from
- Isolation and characterization of multiple forms of malt deoxyribonuclease (1977), Phytochemistry, 16, 1469-1474.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| prolonged storage in the frozen state in H2O causes a gradual decrease of activity, half life of DNases A and B is 2 months | Hordeum vulgare |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| E2-immunity protein | complete inhibition of activity on plasmid DNA | Hordeum vulgare |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for stability | Hordeum vulgare |  |
| Mg2+ | - |
Hordeum vulgare | |
| Mn2+ | - |
Hordeum vulgare | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
SDS-PAGE, gel filtration | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Hordeum vulgare | involved in seed sprouting | 5'-phosphooligonucleotides + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
2 active compounds, A and B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by chromatography steps | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Hordeum vulgare | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Hordeum vulgare | 5'-phosphooligonucleotides + ? | - |
? | |
| double-stranded DNA + H2O | involved in seed sprouting | Hordeum vulgare | 5'-phosphooligonucleotides + ? | - |
? | |
| NO2-Ph-pdTp-NO2-Ph + H2O | - |
Hordeum vulgare | p-nitrophenol + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, SDS-PAGE, gel filtration | Hordeum vulgare |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNase A | - |
Hordeum vulgare |
| DNase B | - |
Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
in the presence of Mg2+, dependent on bivalent metal ions | Hordeum vulgare |
| 7.5 | - |
in the presence of Mn2+, different pH-optima, dependent on bivalent metal ions | Hordeum vulgare |
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