Any feedback?
Literature summary for 3.1.21.1 extracted from
- Purification and properties of human pancreatic deoxyribonuclease I (1977), J. Biochem., 82, 1771-1777.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 80% inhibition at 0.1 mM | Homo sapiens |  |
| EGTA | activity inhibited | Homo sapiens | |
| G-actin | complete inhibition at 0.001 mg | Homo sapiens | |
| iodoacetate | complete inhibition | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secreted into duodenal juice | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation synergistic with Mg2+ | Homo sapiens | |
| Mg2+ | optimal concentration 5 mM | Homo sapiens | |
| Mg2+ | activation synergistic with Ca2+ | Homo sapiens | |
| Mn2+ | 120% activity of Mg2+ activated enzyme at 10 mM | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Homo sapiens | hydrolysis of both double- and single-stranded DNA | 5'-phosphooligonucleotides + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| near homogeneity by fractionation steps | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| duodenal juice | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.033 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM Tris-HCl, pH 7.5, 5 mM CaCl2, 30% polyethylene glycol, more than 3 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
| double-stranded DNA + H2O | hydrolysis of both double- and single-stranded DNA | Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
| heat-denatured DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
| supercoiled plasmid DNA + H2O | - |
Homo sapiens | linear DNA + ? | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
rather stable up to | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
stable for 7 days | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.6 | in 33 mM Tris-HCl buffer | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
