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Literature summary for 3.1.21.1 extracted from
- Enzymes for modifying and labeling DNA and RNA (1987), Methods Enzymol., 152, 94-110.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | preparation of substrates for the nick translation reaction, production of random DNA fragments, analysis of DNA-protein complexes | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation at 1 mM | Bos taurus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Bos taurus | hydrolysis of both double- and single-stranded DNA | 5'-phosphooligonucleotides + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | hydrolysis of both double- and single-stranded DNA | Bos taurus | 5'-phosphooligonucleotides + ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bos taurus |
| 7.5 | - |
- |
Bos taurus |
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