Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | competitive | Rattus norvegicus | |
acetyl-CoA | competitive | Rattus norvegicus | |
DL-3-hydroxybutyryl-CoA | competitive | Rattus norvegicus | |
DL-methylmalonyl-CoA | competitive | Rattus norvegicus | |
isobutyryl-CoA | competitive | Rattus norvegicus | |
malonyl-CoA | competitive | Rattus norvegicus | |
n-valeryl-CoA | competitive | Rattus norvegicus | |
propionyl-CoA | competitive | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
3-hydroxy-2-methylpropanoyl-CoA | - |
Rattus norvegicus | |
0.025 | - |
3-hydroxypropionyl-CoA | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-2-methylpropanoyl-CoA + H2O | Rattus norvegicus | enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
427 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-methylmalonyl-CoA + H2O | - |
Rattus norvegicus | CoA + methylmalonate | - |
? | |
3-hydroxy-2-methylpropanoyl-CoA + H2O | - |
Rattus norvegicus | CoA + 3-hydroxy-2-methylpropanoate | - |
? | |
3-hydroxy-2-methylpropanoyl-CoA + H2O | enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA | Rattus norvegicus | ? | - |
? | |
3-hydroxypropanoyl-CoA + H2O | - |
Rattus norvegicus | CoA + 3-hydroxypropanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
150 | - |
3-hydroxy-2-methylpropanoyl-CoA | - |
Rattus norvegicus | |
266 | - |
3-hydroxy-2-methylpropanoyl-CoA | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
hydrolysis of (S)-methylmalonyl-CoA | Rattus norvegicus |
8 | - |
hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5.0, about 30% of maximal activity, pH 7.0: 65% of maximal activity, hydrolysis of (S)-methylmalonyl-CoA | Rattus norvegicus |
5 | 10.5 | pH 5.0: about 40% of maximal activity, pH 10.5: about 80% of maximal activity, hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA | Rattus norvegicus |