Protein Variants | Comment | Organism |
---|---|---|
D151N | reduced activity | Pseudomonas aeruginosa |
E143A | no detectable activity | Pseudomonas aeruginosa |
E143D | no detectable activity | Pseudomonas aeruginosa |
E143Q | no detectable activity | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-hydroxy-2-methylpropanoyl-CoA + H2O = CoA + 3-hydroxy-2-methylpropanoate | formation of an anhydride reaction intermediate, catalytic mechanism | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyisobutyryl-CoA + H2O | formation of an anhydride reaction intermediate, Glu164 is involved | Pseudomonas aeruginosa | CoA + 3-hydroxyisobutyrate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxyisobutyryl-CoA hydrolase | - |
Pseudomonas aeruginosa |
HICH | - |
Pseudomonas aeruginosa |
More | the enzyme belongs to the crotonase superfamily | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas aeruginosa | |
1 | - |
3-hydroxyisobutyryl-CoA | mutant D151N | Pseudomonas aeruginosa | |
89 | - |
3-hydroxyisobutyryl-CoA | wild-type enzyme, a deuterium isotope of the substrate is used | Pseudomonas aeruginosa |