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Literature summary for 3.1.2.14 extracted from
- Characterization of two acyl-acyl carrier protein thioesterases from developing cuphes seeds specific for medium-chain- and oleoyl-acyl carrier protein (1993), Planta, 189, 425-432.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | - |
Cuphea lanceolata |  |
| (NH4)2SO4 | - |
Cuphea wrightii | |
| NaCl | - |
Cuphea lanceolata | |
| NaCl | - |
Cuphea wrightii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
oleoyl-[acyl-carrier protein] | oleoyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0024 | - |
octanoyl-[acyl-carrier protein] | oleoyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0025 | - |
stearoyl-[acyl-carrier protein] | medium-chain acyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0028 | - |
myristoyl-[acyl-carrier protein] | medium-chain acyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0031 | - |
decanoyl-[acyl-carrier protein] | medium-chain acyl-ACP thioesterase | Cuphea lanceolata | |
| 0.004 | - |
decanoyl-[acyl-carrier protein] | oleoyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0041 | - |
palmitoyl-[acyl-carrier protein] | oleoyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0043 | - |
palmitoyl-[acyl-carrier protein] | medium-chain acyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0049 | - |
dodecanoyl-[acyl-carrier protein] | medium-chain acyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0059 | - |
myristoyl-[acyl-carrier protein] | oleoyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0068 | - |
stearoyl-[acyl-carrier protein] | oleoyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0074 | - |
oleoyl-[acyl-carrier protein] | medium-chain acyl-ACP thioesterase | Cuphea lanceolata | |
| 0.0146 | - |
lauroyl-[acyl-carrier protein] | oleoyl-ACP thioesterase | Cuphea lanceolata | |
| 0.029 | - |
octanoyl-[acyl-carrier protein] | medium-chain acyl-ACP thioesterase | Cuphea lanceolata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
medium-chain acyl-ACP thioesterase, gel filtration | Cuphea lanceolata |
| 53000 | - |
oleoyl-ACP thioesterase, gel filtration | Cuphea lanceolata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cuphea lanceolata | - |
- |
- |
| Cuphea wrightii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two thioesterase activities: medium-chain acyl-ACP thioesterase and oleoyl-ACP thioesterase | Cuphea lanceolata |
| two thioesterase activities: medium-chain acyl-ACP thioesterase and oleoyl-ACP thioesterase | Cuphea wrightii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Cuphea lanceolata | - |
| seed | - |
Cuphea wrightii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0018 | - |
- |
Cuphea lanceolata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| decanoyl-[acyl-carrier protein] + H2O | - |
Cuphea lanceolata | decanoic acid + acyl-carrier protein | - |
? | |
| lauroyl-[acyl-carrier protein] + H2O | activity of the medium-chain acyl-ACP thioesterase | Cuphea lanceolata | lauric acid + acyl-carrier protein | - |
? | |
| lauroyl-[acyl-carrier protein] + H2O | activity of the medium-chain acyl-ACP thioesterase | Cuphea wrightii | lauric acid + acyl-carrier protein | - |
? | |
| myristoyl-[acyl-carrier protein] + H2O | activity of the medium-chain acyl-ACP thioesterase | Cuphea lanceolata | myristic acid + acyl-carrier protein | - |
? | |
| myristoyl-[acyl-carrier protein] + H2O | activity of the medium-chain acyl-ACP thioesterase | Cuphea wrightii | myristic acid + acyl-carrier protein | - |
? | |
| octanoyl-[acyl-carrier protein] + H2O | - |
Cuphea lanceolata | [acyl-carrier protein] + octanoate | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | activity of the oleoyl-ACP thioesterase | Cuphea lanceolata | oleic acid + acyl-carrier protein | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | activity of the oleoyl-ACP thioesterase | Cuphea wrightii | oleic acid + acyl-carrier protein | - |
? | |
| palmitoyl-[acyl-carrier protein] + H2O | - |
Cuphea lanceolata | [acyl-carrier protein] + palmitate | - |
? | |
| stearoyl-[acyl-carrier protein] + H2O | - |
Cuphea lanceolata | [acyl-carrier protein] + stearate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | medium-chain acyl-ACP thioesterase | Cuphea lanceolata |
| 9.3 | - |
oleoyl-ACP thioesterase | Cuphea lanceolata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 9 | medium-chain acyl-ACP thioesterase | Cuphea lanceolata |
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