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Literature summary for 3.1.2.14 extracted from
- Characterization of substrate specificity of plant FatA and FatB acyl-ACP thioesterases (2002), Arch. Biochem. Biophys., 403, 25-34.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of wild-type isozymes and chimeric mutants in Escherichia coli Bl21(DE3), the chimeric mutants are constructed by combination of the N-terminus of FatA with the C-terminus of FatB resulting in FatAB, or vice versa resulting in FatBA | Coriandrum sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km is slightly influenced by substrate chain length and double bonds | Arabidopsis thaliana | |
| additional information | - |
additional information | Km is slightly influenced by substrate chain length and double bonds | Coriandrum sativum | |
| 0.0014 | - |
oleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Spinacia oleracea |  |
| 0.0026 | - |
oleoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.0026 | - |
palmitoleoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.0029 | - |
myristoleoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.003 | - |
stearoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.0031 | - |
oleoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.0038 | - |
palmitoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.004 | - |
myristoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.0049 | - |
palmitoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.0049 | - |
palmitoleoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.005 | - |
stearoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.0089 | - |
myristoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.0139 | - |
myristoleoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid stroma | soluble | Spinacia oleracea | 9532 | - |
| plastid stroma | nuclear gene encoded, targeted to the plastids, soluble | Arabidopsis thaliana | 9532 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-[acyl-carrier protein] + H2O | Spinacia oleracea | - |
oleic acid + acyl-carrier protein | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | Arabidopsis thaliana | - |
oleic acid + acyl-carrier protein | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | Coriandrum sativum | - |
oleic acid + acyl-carrier protein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
isozyme FatA and FatB | - |
| Coriandrum sativum | - |
isozyme FatA | - |
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification | Spinacia oleracea |
| partial purification of the isozymes and the recombinant chimeric mutants | Coriandrum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificities of isozymes and chimeric mutants, kcat is strongly influenced by substrate chain length and double bonds | Coriandrum sativum | ? | - |
? | |
| additional information | the substrate specificity determinants are located at the N-terminus of the isozymes, kcat is strongly influenced by substrate chain length and double bonds | Arabidopsis thaliana | ? | - |
? | |
| myristoleoyl-[acyl-carrier protein] + H2O | - |
Coriandrum sativum | myristoleic acid + acyl-carrier protein | - |
? | |
| myristoleoyl-[acyl-carrier protein] + H2O | low activity | Arabidopsis thaliana | myristoleic acid + acyl-carrier protein | - |
? | |
| myristoyl-[acyl-carrier protein] + H2O | low activity | Arabidopsis thaliana | myristic acid + acyl-carrier protein | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | - |
Spinacia oleracea | oleic acid + acyl-carrier protein | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | - |
Arabidopsis thaliana | oleic acid + acyl-carrier protein | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | - |
Coriandrum sativum | oleic acid + acyl-carrier protein | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | preferred substrate of recombinant chimeric mutant FatAB | Arabidopsis thaliana | oleic acid + acyl-carrier protein | - |
? | |
| palmitoleoyl-[acyl-carrier protein] + H2O | - |
Arabidopsis thaliana | palmitoleic acid + acyl-carrier protein | - |
? | |
| palmitoleoyl-[acyl-carrier protein] + H2O | - |
Coriandrum sativum | palmitoleic acid + acyl-carrier protein | - |
? | |
| palmitoyl-[acyl-carrier protein] + H2O | - |
Coriandrum sativum | palmitic acid + acyl-carrier protein | - |
? | |
| palmitoyl-[acyl-carrier protein] + H2O | preferred substrate of recombinant chimeric mutant FatBA, isozyme FatB prefers [acyl-carrier-protein]-substrate of spinach before the Escherichia coli derived | Arabidopsis thaliana | palmitic acid + acyl-carrier protein | - |
? | |
| stearoyl-[acyl-carrier protein] + H2O | - |
Arabidopsis thaliana | stearic acid + acyl-carrier protein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 12:0-ACP thioesterase | - |
Spinacia oleracea |
| 12:0-ACP thioesterase | - |
Arabidopsis thaliana |
| 12:0-ACP thioesterase | - |
Coriandrum sativum |
| 12:0-acyl-carrier protein thioesterase | - |
Spinacia oleracea |
| 12:0-acyl-carrier protein thioesterase | - |
Arabidopsis thaliana |
| 12:0-acyl-carrier protein thioesterase | - |
Coriandrum sativum |
| 14:0-ACP thioesterase | - |
Spinacia oleracea |
| 14:0-ACP thioesterase | - |
Arabidopsis thaliana |
| 14:0-ACP thioesterase | - |
Coriandrum sativum |
| 14:0-acyl-carrier protein thioesterase | - |
Spinacia oleracea |
| 14:0-acyl-carrier protein thioesterase | - |
Arabidopsis thaliana |
| 14:0-acyl-carrier protein thioesterase | - |
Coriandrum sativum |
| 16:0-ACP thioesterase | - |
Spinacia oleracea |
| 16:0-ACP thioesterase | - |
Arabidopsis thaliana |
| 16:0-ACP thioesterase | - |
Coriandrum sativum |
| 16:0-acyl-carrier protein thioesterase | - |
Spinacia oleracea |
| 16:0-acyl-carrier protein thioesterase | - |
Arabidopsis thaliana |
| 16:0-acyl-carrier protein thioesterase | - |
Coriandrum sativum |
| 18:0-ACP thioesterase | - |
Spinacia oleracea |
| 18:0-ACP thioesterase | - |
Arabidopsis thaliana |
| 18:0-ACP thioesterase | - |
Coriandrum sativum |
| 18:0-acyl-carrier protein thioesterase | - |
Spinacia oleracea |
| 18:0-acyl-carrier protein thioesterase | - |
Arabidopsis thaliana |
| 18:0-acyl-carrier protein thioesterase | - |
Coriandrum sativum |
| acyl-ACP thioesterase | - |
Spinacia oleracea |
| acyl-ACP thioesterase | - |
Arabidopsis thaliana |
| acyl-ACP thioesterase | - |
Coriandrum sativum |
| acyl-ACP-hydrolase | - |
Spinacia oleracea |
| acyl-ACP-hydrolase | - |
Arabidopsis thaliana |
| acyl-ACP-hydrolase | - |
Coriandrum sativum |
| acyl-acyl carrier protein hydrolase | - |
Spinacia oleracea |
| acyl-acyl carrier protein hydrolase | - |
Arabidopsis thaliana |
| acyl-acyl carrier protein hydrolase | - |
Coriandrum sativum |
| acyl-acyl carrier protein thioesterase | - |
Spinacia oleracea |
| acyl-acyl carrier protein thioesterase | - |
Arabidopsis thaliana |
| acyl-acyl carrier protein thioesterase | - |
Coriandrum sativum |
| acyl-[acyl-carrier-protein] hydrolase | - |
Spinacia oleracea |
| acyl-[acyl-carrier-protein] hydrolase | - |
Arabidopsis thaliana |
| acyl-[acyl-carrier-protein] hydrolase | - |
Coriandrum sativum |
| AH | - |
Spinacia oleracea |
| AH | - |
Arabidopsis thaliana |
| AH | - |
Coriandrum sativum |
| BTE | - |
Spinacia oleracea |
| BTE | - |
Arabidopsis thaliana |
| BTE | - |
Coriandrum sativum |
| C12:0 ACP TE | - |
Spinacia oleracea |
| C12:0 ACP TE | - |
Arabidopsis thaliana |
| C12:0 ACP TE | - |
Coriandrum sativum |
| C18:1-ACP thioesterase | - |
Spinacia oleracea |
| C18:1-ACP thioesterase | - |
Arabidopsis thaliana |
| C18:1-ACP thioesterase | - |
Coriandrum sativum |
| Deubiquitinating enzyme 14 | - |
Spinacia oleracea |
| Deubiquitinating enzyme 14 | - |
Arabidopsis thaliana |
| Deubiquitinating enzyme 14 | - |
Coriandrum sativum |
| hydrolase, oleoyl-[acyl carrier protein] | - |
Spinacia oleracea |
| hydrolase, oleoyl-[acyl carrier protein] | - |
Arabidopsis thaliana |
| hydrolase, oleoyl-[acyl carrier protein] | - |
Coriandrum sativum |
| oleoyl-ACP thioesterase | - |
Spinacia oleracea |
| oleoyl-ACP thioesterase | - |
Arabidopsis thaliana |
| oleoyl-ACP thioesterase | - |
Coriandrum sativum |
| oleoyl-acyl carrier protein thioesterase | - |
Spinacia oleracea |
| oleoyl-acyl carrier protein thioesterase | - |
Arabidopsis thaliana |
| oleoyl-acyl carrier protein thioesterase | - |
Coriandrum sativum |
| OTE | - |
Spinacia oleracea |
| OTE | - |
Arabidopsis thaliana |
| OTE | - |
Coriandrum sativum |
| PATE | - |
Spinacia oleracea |
| PATE | - |
Arabidopsis thaliana |
| PATE | - |
Coriandrum sativum |
| STE | - |
Spinacia oleracea |
| STE | - |
Arabidopsis thaliana |
| STE | - |
Coriandrum sativum |
| Ubiquitin thiolesterase 14 | - |
Spinacia oleracea |
| Ubiquitin thiolesterase 14 | - |
Arabidopsis thaliana |
| Ubiquitin thiolesterase 14 | - |
Coriandrum sativum |
| Ubiquitin-specific processing protease 14 | - |
Spinacia oleracea |
| Ubiquitin-specific processing protease 14 | - |
Arabidopsis thaliana |
| Ubiquitin-specific processing protease 14 | - |
Coriandrum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
room temperature, assay at | Spinacia oleracea |
| 22 | - |
room temperature, assay at | Coriandrum sativum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | recombinant chimeric mutants FatAB and FatBA | Arabidopsis thaliana | |
| 0.004 | - |
myristoleoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.008 | - |
myristoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.02 | - |
myristoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.04 | - |
palmitoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.12 | - |
palmitoleoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.15 | - |
stearoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.22 | - |
myristoleoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.74 | - |
palmitoleoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 0.91 | - |
stearoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 1.23 | - |
oleoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 1.94 | - |
oleoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 2.4 | - |
palmitoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 2.94 | - |
oleoyl-[acyl-carrier protein] | isozyme FatA, pH 8.0, 22°C | Arabidopsis thaliana | |
| 6.08 | - |
stearoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 6.08 | - |
oleoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
| 6.08 | - |
palmitoleoyl-[acyl-carrier protein] | isozyme FatB, pH 8.0, 22°C | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Spinacia oleracea |
| 8 | - |
assay at | Coriandrum sativum |
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