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Literature summary for 3.1.13.5 extracted from

  • Zimmer, S.L.; McEvoy, S.M.; Li, J.; Qu, J.; Read, L.K.
    A novel member of the RNase D exoribonuclease family functions in mitochondrial guide RNA metabolism in Trypanosoma brucei (2011), J. Biol. Chem., 286, 10329-10340.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.5

Cloned(Commentary)

Cloned (Comment) Organism
ORF Tb09.211.3670, DNA and amino acid sequence determination and analysis, generation of pRND-MHT for tetracycline-regulated expression of TbRND with a C-terminal myc-His6-TAP tag in Trypanosoma brucei, expression of N-terminally GST- and His-tagged TbRND in Escherichia coli Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
D80A site-directed mutagenesis Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion predicted mitochondrial targeting sequence present at the N-terminus of TbRND Trypanosoma brucei 5739
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma brucei in vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridine homopolymers, including the 3' oligo(U) tails of guide RNAs that provide the sequence information for RNA editing ?
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 ?
additional information Trypanosoma brucei 29-13 in vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridine homopolymers, including the 3' oligo(U) tails of guide RNAs that provide the sequence information for RNA editing ?
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 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q38DE2
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Trypanosoma brucei 29-13 Q38DE2
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Purification (Commentary)

Purification (Comment) Organism
recombinant GST- and His-tagged TbRND from Escherichia coli by sequential affinity chromatography on glutathione and nickel affinity resins Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridine homopolymers, including the 3' oligo(U) tails of guide RNAs that provide the sequence information for RNA editing Trypanosoma brucei ?
-
 ?
additional information substrate is gA6 RNA Trypanosoma brucei ?
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 ?
additional information in vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridine homopolymers, including the 3' oligo(U) tails of guide RNAs that provide the sequence information for RNA editing Trypanosoma brucei 29-13 ?
-
 ?
additional information substrate is gA6 RNA Trypanosoma brucei 29-13 ?
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 ?

Synonyms

Synonyms Comment Organism
RNase D
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 Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
 assay at Trypanosoma brucei

Expression

Organism Comment Expression
Trypanosoma brucei the expression of mitochondrial exoribonuclease TbRND is highly up-regulated in the insect proliferative stage of the parasite up

General Information

General Information Comment Organism
evolution TbRND shares sequence similarity with RNase D family enzymes but differs from all reported members of this family in possessing a CCHC zinc finger domain Trypanosoma brucei
malfunction TbRND depletion results in gRNA tails extended by 2-3 nucleotides on average. Second, overexpression of wild-type but not catalytically inactive TbRND results in a substantial decrease in the total gRNA population and a consequent inhibition of RNA editing. The observed effects on the gRNA population are specific as rRNAs, which are also 3'-uridylated, are unaffected by TbRND depletion or overexpression, phenotypes, overview Trypanosoma brucei
physiological function TbRND functions in guide RNA metabolism in vivo, TbRND is a 3' to 5' exoribonuclease that functions highly specific to the mitochondrion of trypanosomes. In vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridine homopolymers, including the 3' oligo(U) tails of guide RNAs that provide the sequence information for RNA editing Trypanosoma brucei