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Literature summary for 3.1.13.5 extracted from

  • Cudny, H.; Zaniewski, R.; Deutscher, M.P.
    Escherichia coli RNase D. Catalytic properties and substrate specificity (1981), J. Biol. Chem., 256, 5633-5637.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme requires a divalent cation for activity. This requirement can be satisfied by Mg2+, Mn2+ or Co2+ Escherichia coli
Mg2+ enzyme requires a divalent cation for activity. This requirement can be satisfied by Mg2+, Mn2+ or Co2+ Escherichia coli
Mn2+ enzyme requires a divalent cation for activity. This requirement can be satisfied by Mg2+, Mn2+ or Co2+ Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information alteration of the 3'-terminal base has no effect on the rate of hydrolysis, whereas modification of the 3'-terminal sugar has a major effect. tRNA terminating with a 3'-phosphate is completely inactive as a substrate. The rate of hydrolysis of intact tRNA is very slow compared to tRNAs containing extra residues or compared to tRNAs from which part of the -C-C-A sequence has been removed. Oxidation of the terminal sugar, reduction of the dialdehyde with borohydride, or removal of the terminal AMP from intact tRNA increase the activity of the substrate. Addition of a second -C-C-A sequence gives an active substrate indicating that the relative resistance of intact tRNA to RNase D hydrolysis is not due to the sequence per se but to the structural environment of the 3'-terminus. The enzyme is an exonuclease which initiates hydrolysis at the 3'-terminus and removes 5'-mononucleotides in a random fashion Escherichia coli ?
-
?
tRNA-C-C-A-Cn + H2O
-
Escherichia coli tRNA-C-C-A-Cn-1 + cytosine
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.1 9.5
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
8 10.3 pH 8.0: about 50% of maximal activity, pH 10.3: about 70% of maximal activity Escherichia coli