Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | stimulates | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibition can be reversed by Mg2+ | Escherichia coli | |
Na+ | slight | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates | Escherichia coli | |
Mg2+ | required, optimal concentration is 5 mM | Escherichia coli | |
Mn2+ | can partially replace for Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme may represent an intracellular scavenging mechanism for denatured tRNAs and other inactive RNA molecules | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme may represent an intracellular scavenging mechanism for denatured tRNAs and other inactive RNA molecules | Escherichia coli | ? | - |
? | |
structurally altered tRNA | exonuclease activity. RNase D can recognize structurally altered tRNA molecules. The enzyme acts poorly on intact tRNA and is inactive with the synthetic polyribonucleotides, poly(A), poly(U), or double-stranded poly(A)*poly(U). The enzyme acts on diesterase-treated tRNA, but relatively poorly on intact tRNA. RNase D does not attack ribosomal RNA | Escherichia coli | 5'-mononucleotide + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | with diesterase-treated tRNA as substrate | Escherichia coli |