Cloned (Comment) | Organism |
---|---|
gene PARN, expression of His-tagged enzyme in Escherichia coli strain Bl21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L291A | site-directed mutagenesis, activity and kinetics with RNA substrates compared to the wild-type enzyme | Homo sapiens |
M425A | site-directed mutagenesis, activity and kinetics with RNA substrates compared to the wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene PARN | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | synthesis of RNA substrates A5 to A20, G20, C20, U20, AAA, GGG, CCC, UUU, AXX, XXA, XAX, AXA, AAX, and XAA, where X denotes G, C, or U, and usage of commercial substrate, a 44-nucleotide heteropolymeric RNA, with sequence 5'-CCA UCU CAU CCC UGC GUG UCC CAU CUG UUC CCU CCC UGU CUC AG-3', substrate specificity of the recombinant enzyme, overview. The active site of PARN per se harbors specificity for recognition of adenosine | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PARN | - |
Homo sapiens |
poly(A)-specific ribonuclease | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the active site of PARN per se harbors specificity for recognition of adenosine | Homo sapiens |