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Literature summary for 3.1.13.4 extracted from

  • Henriksson, N.; Nilsson, P.; Wu, M.; Song, H.; Virtanen, A.
    Recognition of adenosine residues by the active site of poly(A)-specific ribonuclease (2010), J. Biol. Chem., 285, 163-170.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene PARN, expression of His-tagged enzyme in Escherichia coli strain Bl21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
L291A site-directed mutagenesis, activity and kinetics with RNA substrates compared to the wild-type enzyme Homo sapiens
M425A site-directed mutagenesis, activity and kinetics with RNA substrates compared to the wild-type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
gene PARN
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information synthesis of RNA substrates A5 to A20, G20, C20, U20, AAA, GGG, CCC, UUU, AXX, XXA, XAX, AXA, AAX, and XAA, where X denotes G, C, or U, and usage of commercial substrate, a 44-nucleotide heteropolymeric RNA, with sequence 5'-CCA UCU CAU CCC UGC GUG UCC CAU CUG UUC CCU CCC UGU CUC AG-3', substrate specificity of the recombinant enzyme, overview. The active site of PARN per se harbors specificity for recognition of adenosine Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
PARN
-
Homo sapiens
poly(A)-specific ribonuclease
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Homo sapiens

General Information

General Information Comment Organism
additional information the active site of PARN per se harbors specificity for recognition of adenosine Homo sapiens