Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.13.4 extracted from

  • Utter, C.J.; Garcia, S.A.; Milone, J.; Bellofatto, V.
    Poly(A)-specific ribonuclease (PARN-1) function in stage-specific mRNA turnover in Trypanosoma brucei (2011), Eukaryot. Cell, 10, 1230-1240.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of the GST-tagged C-terminal 133 amino acids of the PARN-1 open reading, cloned into the pGEX-6P-1 bacterial expression vector, in Escherichia coli strain BL21 Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
additional information Cells overexpressing gene PARN-1 have increased deadenylase activity, overexpression of gene PARN-1 leads to PARN-1 affecting the steady-state level and decay rates of at least four different procyclic mRNAs. Depletion of all three PARNs, PARN-1, PARN-2, and PARN-3, using RNA interference in procyclic and bloodstream-form parasites, neither growth rates nor gross morphology are affected in PARN-depleted procyclic forms, and growth rates are only slightly reduced to 90% of control rates in PARN-depleted BF parasites. Following depletion of PARN-1 protein using PARN-1-specific antibodies, RNA-A60 degradation is abolished Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
additional information a poly(A) competitor inhibits RNA-A60 deadenylation by isozyme PARN-1, whereas a poly(C) competitor has no effect on RNA-A60 deadenylation Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Trypanosoma brucei 5737
-
mitochondrion
-
 Trypanosoma brucei 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required Trypanosoma brucei
Mg2+ required Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
 genes PARN-1, PARN-2, and PARN-3
-
Trypanosoma brucei Lister 427
-
 genes PARN-1, PARN-2, and PARN-3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged C-terminal 133 amino acids of the PARN-1 from Escherichia coli strain BL21 Trypanosoma brucei

Source Tissue

Source Tissue Comment Organism Textmining
29-13 cell from Procyclic parasites overexpressing PARN-1 Trypanosoma brucei
-
additional information Trypanosomatids possess three different PARN genes, PARN-1, -2, and -3, each of which is expressed at the mRNA level in two life-cycle stages of the human parasite Trypanosoma brucei Trypanosoma brucei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isozyme PARN-1 degrades RNA-A60. No activity with RNA-A60 with a 22-heteronucleotide sequence added 3' to the poly(A) tail of RNA-A60 as substrate Trypanosoma brucei ?
-
 ?
additional information isozyme PARN-1 degrades RNA-A60. No activity with RNA-A60 with a 22-heteronucleotide sequence added 3' to the poly(A) tail of RNA-A60 as substrate Trypanosoma brucei Lister 427 ?
-
 ?

Synonyms

Synonyms Comment Organism
adenosine-specific exonuclease
-
 Trypanosoma brucei
PARN-1
-
 Trypanosoma brucei
poly(A)-specific ribonuclease
-
 Trypanosoma brucei
poly(A)-specific RNase
-
 Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Trypanosoma brucei

General Information

General Information Comment Organism
physiological function PARN-1 is an active deadenylase, PARN-1 is nonessential for parasite viability Trypanosoma brucei