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Literature summary for 3.1.13.4 extracted from
- Effect of magnesium ions on the thermal stability of human poly(A)-specific ribonuclease (2007), FEBS Lett., 581, 1047-1052.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | Mg2+ has dual effects on PARN stability, protecting the active site against denaturation, but destabilizing the overall structural stability of the protein | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmid pET33 containing the human parn gene expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 3 mM gradually decreases activity about 15fold | Homo sapiens |  |
| Co2+ | 3 mM gradually decreases activity about 78fold | Homo sapiens | |
| Mg2+ | behaves as a destabilizer of the overall structural stability of PARN, promotes thermal unfolding and aggregation at high temperatures | Homo sapiens | |
| Mn2+ | 3 mM gradually decreases activity about 5fold | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | can protect PARN against thermal inactivation by increasing the midpoint of inactivation and decreasing the inactivation rate, protective effect is unique to Mg2+ in a concentration-dependent manner | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni2+ affinity chromatography and gel filtration, purity above 98% | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(A) RNA + H2O | - |
Homo sapiens | 5'-AMP + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PARN | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 49 | - |
in the absence of Mg2+, the activity of PARN decreases continuously with increasing temperature | Homo sapiens |
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