Cloned (Comment) | Organism |
---|---|
his-tagged recombinant wild type and mutants | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D28C | mutants inactive in presence of Mg2+, but with restored activity in presence of soft divalent metal ions, amino acid interacts with catalytically essential metal ions | Escherichia coli |
D292C | mutants inactive in presence of Mg2+, but with restored activity in presence of soft divalent metal ions, amino acid interacts with catalytically essential metal ions | Escherichia coli |
D382C | mutants inactive in presence of Mg2+, but with restored activity in presence of soft divalent metal ions, amino acid interacts with catalytically essential metal ions | Escherichia coli |
E30C | inactive mutant, acitivity cannot be restored | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 2 mM, activates reaction, can modulated the substrate length requirement | Escherichia coli | |
Co2+ | 2 mM, activates reaction, can modulated the substrate length requirement | Escherichia coli | |
Mg2+ | 2 mM, activates reaction, can modulated the substrate length requirement | Escherichia coli | |
Mn2+ | 2 mM, activates reaction | Escherichia coli | |
Zn2+ | 2 mM, activates reaction | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli BL21 (DE3) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ matrix | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PARN | - |
Escherichia coli |