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Literature summary for 3.1.13.4 extracted from

  • Reverdatto, S.V.; Dutko, J.A.; Chekanova, J.A.; Hamilton, D.A.; Belostotsky, D.A.
    mRNA deadenylation by PARN is essential for embryogenesis in higher plants (2004), RNA, 10, 1200-1214.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.4

Cloned(Commentary)

Cloned (Comment) Organism
2 genes, expression and sequence analysis, genotyping of plants, expression of His-tagged wild-type and mutant PARN as functional C-terminal maltose-binding protein-fusion protein in Escherichia coli, expression of wild-type GST-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D66A/E68A site-directed mutagenesis, enzymatically inactive PARN, the mutant embryos are retarded, and culminate in an arrest at the bent-cotyledon stage, some show hyperadenylation of transcripts Arabidopsis thaliana
additional information construction of a PARN disruption mutant, the mutant shows no phenotype Schizosaccharomyces pombe
additional information construction of embryos lacking PARN, the mutant embryos are retarded, and culminate in an arrest at the bent-cotyledon stage, some show hyperadenylation of transcripts, the phenotype cannot be rescued by expression of the wild-type enzyme in plants, loss of the enzyme affects poly(A) tail length distribution of only a select subset of embryonic transcripts Arabidopsis thaliana
additional information gene silencing by microinjection of specific iRNA, the mutant worms show no phenotype Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm predominantly, PARN lacks NLS motifs Arabidopsis thaliana 5737
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana mRNA deadenylation by exoribonucleolytic activity of PARN is essential for embryogenesis and viability, deadenylation of mRNA is often the first and rate-limiting step in mRNA decay, loss of the enzyme affects poly(A) tail length distribution of only a select subset of embryonic transcripts ?
-
 ?
additional information Schizosaccharomyces pombe mRNA deadenylation by exoribonucleolytic activity of PARN is not essential for embryogenesis and viability ?
-
 ?
additional information Caenorhabditis elegans mRNA deadenylation by exoribonucleolytic activity of PARN is not essential for embryogenesis and viability ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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-
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Caenorhabditis elegans
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-
-
Schizosaccharomyces pombe
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
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stem
-
 Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information mRNA deadenylation by exoribonucleolytic activity of PARN is essential for embryogenesis and viability, deadenylation of mRNA is often the first and rate-limiting step in mRNA decay, loss of the enzyme affects poly(A) tail length distribution of only a select subset of embryonic transcripts Arabidopsis thaliana ?
-
 ?
additional information mRNA deadenylation by exoribonucleolytic activity of PARN is not essential for embryogenesis and viability Schizosaccharomyces pombe ?
-
 ?
additional information mRNA deadenylation by exoribonucleolytic activity of PARN is not essential for embryogenesis and viability Caenorhabditis elegans ?
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the family of RNAse D nucleases Arabidopsis thaliana
More the enzyme belongs to the family of RNAse D nucleases Schizosaccharomyces pombe
More the enzyme belongs to the family of RNAse D nucleases Caenorhabditis elegans
PARN
-
 Arabidopsis thaliana
PARN
-
 Schizosaccharomyces pombe
PARN
-
 Caenorhabditis elegans
poly(A)-specific 3'-5' ribonuclease
-
 Arabidopsis thaliana
poly(A)-specific 3'-5' ribonuclease
-
 Schizosaccharomyces pombe
poly(A)-specific 3'-5' ribonuclease
-
 Caenorhabditis elegans