Inhibitors | Comment | Organism | Structure |
---|---|---|---|
m7G(5')ppp(5')G cap analogue | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required, optimal around 100 mM | Bos taurus | |
Mg2+ | divalent cation required around 1 mM, Mg2+ being most effective | Bos taurus | |
Mn2+ | may partly substitute for Mg2+, 17% of activity detected with Mg2+ | Bos taurus | |
additional information | no activtiy with Zn2+, Ca2+ | Bos taurus | |
Na+ | may substitute for K*, less effective | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
3 * 74000, SDS-PAGE, a 54000 Da polypeptide fragment is responsible for activity | Bos taurus |
180000 | 220000 | gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
enzyme and 54 kDa fragment of enzyme | - |
Purification (Comment) | Organism |
---|---|
partial | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
211000 | - |
pH 7.0, 30°C | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A)-mRNA + H2O | - |
Bos taurus | 5'-AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 74000, SDS-PAGE, a 54000 Da polypeptide fragment is responsible for activity | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bos taurus |