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Literature summary for 3.1.1.8 extracted from

  • Xue, L.; Ko, M.; Tong, M.; Yang, W.; Hou, S.; Fang, L.; Liu, J.; Zheng, F.; Woods, J.; Tai, H.; Zhan, C.
    Design, preparation, and characterization of high-activity mutants of human butyrylcholinesterase specific for detoxification of cocaine (2011), Mol. Pharmacol., 79, 290-297.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
wild type and mutant enzymes are expressed in HEK-293T/17 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
A199S/F227A/S287G/A328W/E441D the mutant has significantly lower kcat and KM values against acetylcholine than the wild type enzyme Homo sapiens
A199S/F227A/S287G/A328W/Y332G/E441D the mutant has significantly lower kcat and KM values against acetylcholine than the wild type enzyme Homo sapiens
A199S/S287G/A328W/Y332G the mutant has significantly lower kcat and KM values against acetylcholine than the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
Acetylcholine mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C Homo sapiens
0.036
-
Acetylcholine mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C Homo sapiens
0.075
-
Acetylcholine mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C Homo sapiens
0.148
-
Acetylcholine wild type enzyme, pH not specified in the publication, at 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
QFF resin anion-exchange chromatography and Hypatite C column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
Homo sapiens choline + acetate
-
?

Synonyms

Synonyms Comment Organism
BChE
-
Homo sapiens
butyrylcholinesterase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
125
-
Acetylcholine mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C Homo sapiens
230
-
Acetylcholine mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C Homo sapiens
243
-
Acetylcholine mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C Homo sapiens
1433
-
Acetylcholine wild type enzyme, pH not specified in the publication, at 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3000
-
Acetylcholine mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C Homo sapiens
3500
-
Acetylcholine mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C Homo sapiens
9000
-
Acetylcholine mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C Homo sapiens
9667
-
Acetylcholine wild type enzyme, pH not specified in the publication, at 25°C Homo sapiens