Cloned (Comment) | Organism |
---|---|
wild type and mutant enzymes are expressed in HEK-293T/17 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A199S/F227A/S287G/A328W/E441D | the mutant has significantly lower kcat and KM values against acetylcholine than the wild type enzyme | Homo sapiens |
A199S/F227A/S287G/A328W/Y332G/E441D | the mutant has significantly lower kcat and KM values against acetylcholine than the wild type enzyme | Homo sapiens |
A199S/S287G/A328W/Y332G | the mutant has significantly lower kcat and KM values against acetylcholine than the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
0.036 | - |
Acetylcholine | mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C | Homo sapiens | |
0.075 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
0.148 | - |
Acetylcholine | wild type enzyme, pH not specified in the publication, at 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
QFF resin anion-exchange chromatography and Hypatite C column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Homo sapiens | choline + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BChE | - |
Homo sapiens |
butyrylcholinesterase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
125 | - |
Acetylcholine | mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C | Homo sapiens | |
230 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
243 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
1433 | - |
Acetylcholine | wild type enzyme, pH not specified in the publication, at 25°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3000 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3500 | - |
Acetylcholine | mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C | Homo sapiens | |
9000 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
9667 | - |
Acetylcholine | wild type enzyme, pH not specified in the publication, at 25°C | Homo sapiens |