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Literature summary for 3.1.1.8 extracted from
- The butyrylcholinesterase K-variant shows similar cellular protein turnover and quaternary interaction to the wild-type enzyme (2000), J. Neurochem., 74, 869-877.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in CHO cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A539T | K-variant | Homo sapiens |
| E497V/A539T | J-variant | Homo sapiens |
| E797V | catalytic activity similar to wild-type enzyme | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| half-life of wild-type enzyme in culture: 1.41h, half-life of A539T mutant enzyme in culture: 1.55h, half-life of E497V/A539T mutant enzyme in culture: 1.49h | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
Benzoylcholine | wild-type and A539T mutant, pH 7, 25°C | Homo sapiens |  |
| 0.009 | - |
Benzoylcholine | E497V/A539T mutant, pH 7, 25°C | Homo sapiens | |
| 0.011 | - |
Benzoylcholine | E497V mutant, pH 7, 25°C | Homo sapiens | |
| 0.017 | - |
butyrylthiocholine | A539T mutant, E497V/A539T mutant and E497V mutant, pH 7, 25°C | Homo sapiens | |
| 0.023 | - |
butyrylthiocholine | wild-type, pH 7, 25°C | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoylcholine + H2O | - |
Homo sapiens | choline + benzoate | - |
? | |
| butyrylthiocholine + H2O | - |
Homo sapiens | thiocholine + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | in the presence of poly-L-proline | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 223 | - |
Benzoylcholine | E497V/A539T mutant, pH 7, 25°C | Homo sapiens | |
| 242 | - |
Benzoylcholine | wild-type, pH 7, 25°C | Homo sapiens | |
| 265 | - |
Benzoylcholine | A539T mutant, pH 7, 25°C | Homo sapiens | |
| 303 | - |
Benzoylcholine | E497V mutant, pH 7, 25°C | Homo sapiens | |
| 508 | - |
butyrylthiocholine | E497V/A539T mutant, pH 7, 25°C | Homo sapiens | |
| 542 | - |
butyrylthiocholine | A539T mutant and E497V mutant, pH 7, 25°C | Homo sapiens | |
| 565 | - |
butyrylthiocholine | wild-type, pH 7, 25°C | Homo sapiens | |
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