Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | - |
Thermus thermophilus | |
methanol | - |
Thermus thermophilus | |
additional information | no effect by 2-mercaptoethanol | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethanol | slight inhibition | Thermus thermophilus | |
additional information | no effect by 2-mercaptoethanol on enzyme activity | Thermus thermophilus | |
PMSF | - |
Thermus thermophilus | |
Triton X-100 | inhibition at high concentration | Thermus thermophilus | |
Tween 20 | inhibition at high concentration | Thermus thermophilus | |
Tween 80 | inhibition at high concentration | Thermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Thermus thermophilus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(3-hydroxydecanoate-co-3-hydroxhyoctanoate) + H2O | Thermus thermophilus | - |
? | - |
? | |
poly(3-hydroxydecanoate-co-3-hydroxhyoctanoate) + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
? | - |
? | |
poly(3-hydroxyoctanoic acid) + H2O | Thermus thermophilus | - |
? | - |
? | |
poly(3-hydroxyoctanoic acid) + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
the enzyme is encoded in parts of gene TTHA1605 | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
the enzyme is encoded in parts of gene TTHA1605 | - |
Purification (Comment) | Organism |
---|---|
the enzyme is purified from cell medium 10.4fold by acetone precipitation, hydrophobic interaction chromatography using Octyl-Sepharose CL-4B, and gel filtration to homogeneity | Thermus thermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | cell growth on sodium octanoate | Thermus thermophilus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl octanoate + H2O | - |
Thermus thermophilus | octanoate + 4-nitrophenol | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | octanoate + 4-nitrophenol | - |
? | |
additional information | substrate specificity, overview, the extracellular mcl-PHA depolymerase is also capable of hydrolyzing various short- and medium-chain length 4-nitrophenyl-alkanoates, no activity with poly(3-hydroxybutyrate) | Thermus thermophilus | ? | - |
? | |
additional information | substrate specificity, overview, the extracellular mcl-PHA depolymerase is also capable of hydrolyzing various short- and medium-chain length 4-nitrophenyl-alkanoates, no activity with poly(3-hydroxybutyrate) | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
poly(3-hydroxydecanoate-co-3-hydroxhyoctanoate) + H2O | - |
Thermus thermophilus | ? | - |
? | |
poly(3-hydroxydecanoate-co-3-hydroxhyoctanoate) + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
poly(3-hydroxyoctanoic acid) + H2O | - |
Thermus thermophilus | ? | - |
? | |
poly(3-hydroxyoctanoic acid) + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
MCL-PHA depolymerase | - |
Thermus thermophilus |
medium-chain length polyhydroxyalkanoate depolymerase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
with substrate 4-nitrophenyl octanoate | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | the purified enzyme is most active at pH range of pH 7.0-9.0, while the maximal activity is observed at pH 8.5. At pH 6.0 and pH 11 the enzyme shows 34% and 7.5% of maximal activity, respectively | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
additional information | the mcl-PHA depolymerase from Thermus thermophilus is a distinct enzyme, which is different from those of other mcl-PHAdegrading bacteria | Thermus thermophilus |