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Literature summary for 3.1.1.7 extracted from
- Heterologous expression, purification, and biochemical characterization of a greenbug (Schizaphis graminum) acetylcholinesterase encoded by a paralogous gene (ace-1) (2010), J. Biochem. Mol. Toxicol., 24, 51-59.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| choline | the activity gradually increases from 100% at 0.1 mM to around 120% at 10-20 mM | Schizaphis graminum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in recombinant baculovirus-infectes Sf 9 cells | Schizaphis graminum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| BW284c51 | - |
Schizaphis graminum | |
| Carbaryl | - |
Schizaphis graminum | |
| choline | the activity decreases to 40% at 200 mM | Schizaphis graminum | |
| ethopropazine | - |
Schizaphis graminum | |
| malaoxon | - |
Schizaphis graminum | |
| paraoxon | - |
Schizaphis graminum | |
| serine | - |
Schizaphis graminum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0689 | - |
propionylthiocholine iodide | pH and temperature not specified in the publication | Schizaphis graminum | |
| 0.0883 | - |
acetylthiocholine iodide | pH and temperature not specified in the publication | Schizaphis graminum | |
| 0.1139 | - |
acetyl(beta-methyl)thiocholine iodide | pH and temperature not specified in the publication | Schizaphis graminum | |
| 0.2011 | - |
(S)-butyrylthiocholine iodide | pH and temperature not specified in the publication | Schizaphis graminum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64597 | - |
2 * 64597, calculated from amino acid sequence | Schizaphis graminum |
| 70000 | - |
2 * 70000, SDS-PAGE | Schizaphis graminum |
| 130000 | - |
nonreducing PAGE | Schizaphis graminum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizaphis graminum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| dextran sulfate-Sepharose column chromatography and Ni-NTA column chromatography | Schizaphis graminum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl(beta-methyl)thiocholine iodide + H2O | - |
Schizaphis graminum | 1-methyl(thio)choline iodide + acetate | - |
? | |
| acetylthiocholine iodide + H2O | - |
Schizaphis graminum | thiocholine iodide + acetate | - |
? | |
| propionylthiocholine iodide + H2O | - |
Schizaphis graminum | thiocholine iodide + propionate | - |
? | |
| S-butyrylthiocholine iodide + H2O | - |
Schizaphis graminum | thiocholine iodide + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 70000, SDS-PAGE | Schizaphis graminum |
| homodimer | 2 * 64597, calculated from amino acid sequence | Schizaphis graminum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACE-1 | - |
Schizaphis graminum |
| acetylcholinesterase-1 | - |
Schizaphis graminum |
| AChE1 | - |
Schizaphis graminum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 25 | - |
Schizaphis graminum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000883 | - |
(S)-butyrylthiocholine iodide | pH and temperature not specified in the publication | Schizaphis graminum | |
| 0.0000825 | - |
propionylthiocholine iodide | pH and temperature not specified in the publication | Schizaphis graminum | |
| 0.000143 | - |
acetylthiocholine iodide | pH and temperature not specified in the publication | Schizaphis graminum | |
| 0.000146 | - |
acetyl(beta-methyl)thiocholine iodide | pH and temperature not specified in the publication | Schizaphis graminum | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | - |
Schizaphis graminum |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.000016 | - |
pH and temperature not specified in the publication | Schizaphis graminum | paraoxon | |
| 0.000061 | - |
pH and temperature not specified in the publication | Schizaphis graminum | malaoxon | |
| 0.0002 | - |
pH and temperature not specified in the publication | Schizaphis graminum | serine | |
| 0.00094 | - |
pH and temperature not specified in the publication | Schizaphis graminum | Carbaryl | |
| 0.00166 | - |
pH and temperature not specified in the publication | Schizaphis graminum | BW284c51 | |
| 0.102 | - |
pH and temperature not specified in the publication | Schizaphis graminum | ethopropazine | |
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