Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris and in HEK-293 cells | Ctenocephalides felis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Carbaryl | i.e. N-methyl-1-naphthylcarbamate, complete inhibition at concentrations exceeding 100 nM; i.e. N-methyl-1-naphthylcarbamate, complete inhibition at concentrations exceeding 100 nM | Ctenocephalides felis | |
galanthamine | - |
Ctenocephalides felis | |
propoxur | i.e. 2-isopropoxyphenyl-N-methylcarbamate, complete inhibition at concentrations exceeding 100 nM; i.e. 2-isopropoxyphenyl-N-methylcarbamate, complete inhibition at concentrations exceeding 100 nM | Ctenocephalides felis | |
tacrine | - |
Ctenocephalides felis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0158 | - |
propionylthiocholine | isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | |
0.0243 | - |
acetylthiocholine | isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | |
0.0244 | - |
butyrylthiocholine | isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | |
0.0376 | - |
propionylthiocholine | isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | |
0.0484 | - |
acetylthiocholine | isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | |
0.0684 | - |
butyrylthiocholine | isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | isoform AChE1 possesses glycosylphosphatidylinositol membrane anchors and are transported to the cell surface | Ctenocephalides felis | 9986 | - |
cell surface | isoform AChE2 possesses glycosylphosphatidylinositol membrane anchors and is transported to the cell surface | Ctenocephalides felis | 9986 | - |
membrane | the majority (81%) of the AChE activity in fleas is membrane-bound | Ctenocephalides felis | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ctenocephalides felis | D2U6X5 | cat flea | - |
Ctenocephalides felis | D2U6X6 | cat flea | - |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography | Ctenocephalides felis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine + H2O | - |
Ctenocephalides felis | thiocholine + acetate | - |
? | |
butyrylthiocholine + H2O | - |
Ctenocephalides felis | thiocholine + butyrate | - |
? | |
additional information | isoform AChE1 is the dominant activity in Ctenocephalides felis imagoes | Ctenocephalides felis | ? | - |
? | |
propionylthiocholine + H2O | - |
Ctenocephalides felis | thiocholine + propionate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AChE1 | isoform | Ctenocephalides felis |
AChE2 | isoform | Ctenocephalides felis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000009 | - |
isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | Carbaryl | |
0.000012 | - |
isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | propoxur | |
0.000024 | - |
isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | propoxur | |
0.000038 | - |
isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | Carbaryl | |
0.000126 | - |
isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | tacrine | |
0.00208 | - |
isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C | Ctenocephalides felis | tacrine |