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Literature summary for 3.1.1.7 extracted from
- Thermal denaturation of wild type and mutant recombinant acetylcholinesterase from amphioxus: effects of the temperature of in vitro expression and of reversible inhibitors (2008), Invert. Neurosci., 8, 147-155.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in COS-7 cells | Branchiostoma floridae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C310A | site-directed mutagenesis, the mutant shows altered thermal stability and sensitivity to inhibitors compared to the wild-type enzyme | Branchiostoma floridae |
| C310A/C466A | site-directed mutagenesis, the mutant shows altered thermal stability and sensitivity to inhibitors compared to the wild-type enzyme | Branchiostoma floridae |
| C310A/F312I | site-directed mutagenesis, the mutant shows unaltered thermal stability but altered sensitivity to inhibitors compared to the wild-type enzyme | Branchiostoma floridae |
| C466A | site-directed mutagenesis, the mutant shows altered thermal stability and sensitivity to inhibitors compared to the wild-type enzyme | Branchiostoma floridae |
| F312I | site-directed mutagenesis, the mutant shows altered thermal stability and sensitivity to inhibitors compared to the wild-type enzyme | Branchiostoma floridae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| BW284c51 | - |
Branchiostoma floridae |  |
| edrophonium | - |
Branchiostoma floridae | |
| ethopropazine | - |
Branchiostoma floridae | |
| propidium | - |
Branchiostoma floridae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Branchiostoma floridae | - |
- |
- |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure homology molecular modelling, overview | Branchiostoma floridae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChE2 | - |
Branchiostoma floridae |
| cholinesterase 2 | - |
Branchiostoma floridae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | assay at | Branchiostoma floridae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | thermal inactivation rates and kinetics of wild-type and mutant enzymes, overview | Branchiostoma floridae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Branchiostoma floridae |
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Release 2023.1 (January 2023)
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