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Literature summary for 3.1.1.7 extracted from
- Amino acid residues involved in stereoselective inhibition of cholinesterases with bambuterol (2008), Arch. Biochem. Biophys., 471, 72-76.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F295L/Y337A | site-directed mutagenesis, the mutant an altered inhibition rate with inhibitor bambuterol compared to the wild-type enzyme | Mus musculus |
| F295L/Y337A | site-directed mutagenesis, the mutant shows a 13fold increased inhibition rate with inhibitor bambuterol compared to the wild-type enzyme | Mus musculus |
| F296L/F297I/Y337A | site-directed mutagenesis, the mutant shows a 14fold decreased inhibition rate with inhibitor bambuterol compared to the wild-type enzyme | Mus musculus |
| F297I/Y337A | site-directed mutagenesis, the mutant shows an altered inhibition rate with inhibitor bambuterol compared to the wild-type enzyme | Mus musculus |
| Y124Q | site-directed mutagenesis, the mutant shows an altered inhibition rate with inhibitor bambuterol compared to the wild-type enzyme | Mus musculus |
| Y337A | site-directed mutagenesis, the mutant shows an altered inhibition rate with inhibitor bambuterol compared to the wild-type enzyme | Mus musculus |
| Y72N/Y124Q/Y337A | site-directed mutagenesis, the mutant shows an altered inhibition rate with inhibitor bambuterol compared to the wild-type enzyme | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bambuterol | a specific and stereoselective inhibitor of butyrylcholinesterase, which is about 8000times faster inhibited than acetylcholinesterase. AChE wild-type enzyme, and mutants F297I/Y337A and F295L/F297I/Y337A show a deviation from linearity, either enzymes are inhibited by racemate or enantiomers, indicating the presence of reversible enzyme-inhibitor complex, overview | Mus musculus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | P21836 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylthiocholine + H2O | - |
Mus musculus | acetate + thiocholine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AChE | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics, wild-type and mutant enzymes, overview | Mus musculus |
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Release 2023.1 (January 2023)
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