Cloned (Comment) | Organism |
---|---|
stable expression of mutant enzyme in CHO cells | Homo sapiens |
stable expression of wild-type and mutant enzymes in CHO-K1 cells | Bungarus fasciatus |
Protein Variants | Comment | Organism |
---|---|---|
G117H | site-directed mutagenesis, the mutant enzyme hydrolyzes organophosphorous compounds in contrast to the wild-type enzyme | Homo sapiens |
G122H/Y124Q/S125T | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, but does not show substrate inhibition up to 80 mM acetylthiocholine concentration, the mutant enzyme hydrolyzes organophosphorous compounds in contrast to the wild-type enzyme | Bungarus fasciatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chlorpyrifos-oxon | complete inhibition of wild-type enzyme | Bungarus fasciatus | |
demeton | complete inhibition of wild-type enzyme | Bungarus fasciatus | |
diisopropyl phosphofluoridate | complete inhibition of wild-type enzyme, not of mutant G122H/Y124Q/S125T | Bungarus fasciatus | |
Echothiophate | complete inhibition of wild-type enzyme, not of mutant G122H/Y124Q/S125T | Bungarus fasciatus | |
additional information | inhibition mechanism, overview | Bungarus fasciatus | |
paraoxon | complete inhibition of wild-type enzyme, not of mutant G122H/Y124Q/S125T | Bungarus fasciatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, wild-type and mutant enzymes | Bungarus fasciatus | |
0.052 | - |
acetylthiocholine | pH 7.0, 25°C, wild-type enzyme | Bungarus fasciatus | |
34 | - |
acetylthiocholine | pH 7.0, 25°C, mutant G122H/Y124Q/S125T | Bungarus fasciatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bungarus fasciatus | - |
- |
- |
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bungarus fasciatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine + H2O | - |
Homo sapiens | thiocholine + acetate | - |
? | |
acetylthiocholine + H2O | - |
Bungarus fasciatus | thiocholine + acetate | - |
? | |
diisopropyl phosphofluoridate + H2O | mutant G117H, no activity with the wild-type enzyme | Homo sapiens | ? | - |
? | |
diisopropyl phosphofluoridate + H2O | mutant G122H/Y124Q/S125T, no activity with the wild-type enzyme | Bungarus fasciatus | ? | - |
? | |
echothiophate + H2O | mutant G117H, no activity with the wild-type enzyme | Homo sapiens | ? | - |
? | |
echothiophate + H2O | mutant G122H/Y124Q/S125T, no activity with the wild-type enzyme | Bungarus fasciatus | ? | - |
? | |
paraoxon + H2O | mutant G117H, no activity with the wild-type enzyme | Homo sapiens | diethylphosphate + 4-nitrophenol | - |
? | |
paraoxon + H2O | mutant G122H/Y124Q/S125T, no activity with the wild-type enzyme | Bungarus fasciatus | diethylphosphate + 4-nitrophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Homo sapiens |
AChE | - |
Bungarus fasciatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
25 | - |
assay at | Bungarus fasciatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
64.8 | - |
acetylthiocholine | pH 7.0, 25°C, mutant G122H/Y124Q/S125T | Bungarus fasciatus | |
293.3 | - |
acetylthiocholine | pH 7.0, 25°C, mutant G122H/Y124Q/S125T | Bungarus fasciatus | |
4417 | - |
acetylthiocholine | pH 7.0, 25°C, wild-type enzyme | Bungarus fasciatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
7 | - |
assay at | Bungarus fasciatus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Bungarus fasciatus |